Recombinant Anti-CXCR3 antibody [EPR23845-44] (ab259865)
![Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] (ab259865)](https://www.abcam.com/ps/products/259/ab259865/Images/ab259865-1-anti-cxcr3-antibody-epr2384544-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg "Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] (ab259865)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23845-44] to CXCR3
- Suitable for: Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CXCR3 antibody [EPR23845-44]
See all CXCR3 primary antibodies -
Description
Rabbit monoclonal [EPR23845-44] to CXCR3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC,IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: Human peripheral blood mononuclear cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23845-44 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab259865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/500.
|
| Notes |
|---|
|
Flow Cyt
1/500. |
Target
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Function
Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
Isoform 3 mediates the activity of CXCL11. -
Tissue specificity
Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2833 Human
- Omim: 300574 Human
- SwissProt: P49682 Human
- Unigene: 198252 Human
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Alternative names
- C-X-C chemokine receptor type 3 antibody
- CD 183 antibody
- CD182 antibody
see all
Images
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Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] (ab259865)
Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) (treated with 200U/ml Interleukin-2 (IL-2) and 1µg/ml Phytohemagglutinin (PHA) for 6 days) labelling CXCR3 with ab259865 at 1/500 dilution (0.1µg) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or ab259865. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.
Gated on viable cells.
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![Anti-CXCR3 antibody [EPR23845-44] (ab259865)](https://www.abcam.com/ps/products/259/ab259865/Images/ab259865-2-benefits-of-recombinant-antibodies.png)
Anti-CXCR3 antibody [EPR23845-44] (ab259865)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab259865 has not yet been referenced specifically in any publications.
