Overview

  • Product name
    Anti-CXCR3 antibody [EPR7469(B)]
    See all CXCR3 primary antibodies
  • Description
    Rabbit monoclonal [EPR7469(B)] to CXCR3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CXCR3 aa 250-350. The exact sequence is proprietary.

  • Positive control
    • Recombinant Human CXCR3 protein (ab152433) can be used as a positive control in WB. K562, HeLa and HuT-78 cell lysates; K562 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
      Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
      Isoform 3 mediates the activity of CXCL11.
    • Tissue specificity
      Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-X-C chemokine receptor type 3 antibody
      • CD 183 antibody
      • CD182 antibody
      • CD183 antibody
      • Chemokine (C X C motif) receptor 3 antibody
      • Chemokine (C X C) receptor 3 antibody
      • Chemokine CXC Motif Receptor 3 antibody
      • CKR L2 antibody
      • CKR-L2 antibody
      • CKRL2 antibody
      • CMKAR3 antibody
      • CXC-R3 antibody
      • CXCR-3 antibody
      • CXCR3 antibody
      • CXCR3_HUMAN antibody
      • G Protein Coupled Receptor 9 antibody
      • G protein-coupled receptor 9 antibody
      • GPR9 antibody
      • Interferon-inducible protein 10 receptor antibody
      • IP-10 receptor antibody
      • IP10 antibody
      • IP10 R antibody
      • IP10 receptor antibody
      • IP10-R antibody
      • IP10R antibody
      • Mig R antibody
      • Mig receptor antibody
      • Mig-R antibody
      • MIGR antibody
      see all

    Images

    • All lanes : Anti-CXCR3 antibody [EPR7469(B)] (ab154845) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HuT-78 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 41 kDa

    • Immunofluorescence analysis of K562 cells, labeling CXCR3 using ab154845 at a 1/100 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Hueso L  et al. Upregulation of angiostatic chemokines IP-10/CXCL10 and I-TAC/CXCL11 in human obesity and their implication for adipose tissue angiogenesis. Int J Obes (Lond) 42:1406-1417 (2018). Read more (PubMed: 29795466) »
    • Boyé K  et al. The role of CXCR3/LRP1 cross-talk in the invasion of primary brain tumors. Nat Commun 8:1571 (2017). Read more (PubMed: 29146996) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jan 13 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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