Recombinant Anti-CXCR5 antibody [EPR8837] (Alexa Fluor® 647) (ab223979)

Rabbit recombinant monoclonal CXCR5 antibody [EPR8837] conjugated to Alexa Fluor® 647. Validated in Flow Cyt, ICC/IF and tested in Human.


  • Product name

    Anti-CXCR5 antibody [EPR8837] (Alexa Fluor® 647)
    See all CXCR5 primary antibodies
  • Description

    Rabbit monoclonal [EPR8837] to CXCR5 (Alexa Fluor® 647)
  • Host species

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human CXCR5 aa 350-450 (C terminal).
    Database link: P32302

  • Positive control

    • ICC/IF: Raji cells. Flow Cyt: Raji cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab223979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/5000.
ICC/IF 1/1000.

This product gave a positive signal in Raji cells fixed with 4% formaldehyde (10 min) and 80% methanol (5 min)


  • Function

    Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
  • Tissue specificity

    Expression in mature B-cells and Burkitt lymphoma cells.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BLR 1 antibody
    • BLR1 antibody
    • Burkitt lymphoma receptor 1 antibody
    • Burkitt lymphoma receptor 1 GTP binding protein antibody
    • Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5) antibody
    • C X C chemokine receptor type 5 antibody
    • C-X-C chemokine receptor type 5 antibody
    • CD 185 antibody
    • CD185 antibody
    • CD185 antigen antibody
    • Chemokine (C-X-C motif) receptor 5 antibody
    • Chemokine C X C motif receptor 5 antibody
    • Chemokine CXC motif receptor 5 antibody
    • Chemokine receptor 5 antibody
    • CXC chemokine receptor type 5 antibody
    • CXC R5 antibody
    • CXC-R5 antibody
    • CXCR 5 antibody
    • CXCR-5 antibody
    • Cxcr5 antibody
    • CXCR5_HUMAN antibody
    • MDR 15 antibody
    • MDR-15 antibody
    • MDR15 antibody
    • MGC117347 antibody
    • Monocyte derived receptor 15 antibody
    • Monocyte-derived receptor 15 antibody
    see all


  • Overlay histogram showing Raji cells stained with ab223979 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223979, 1/5000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

  • ab223979 staining CXCR5 in Raji cells. The cells were fixed with 80% methanol (5 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab223979 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in Raji cells fixed with 4% formaldehyde (10 min).


ab223979 has not yet been referenced specifically in any publications.

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