- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-CXCR6 antibody
See all CXCR6 primary antibodies -
DescriptionRabbit polyclonal to CXCR6
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide, corresponding to amino acids 11-25 of Human Bonzo.
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Positive control
- Human spleen tissue lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium azide
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Concentration information loading... -
PurityAffinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab8023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| Flow Cyt | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P | Use a concentration of 20 µg/ml. | |
| WB | 1/500. Predicted molecular weight: 44 kDa.Can be blocked with CXCR6 peptide (ab8390). |
Target
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FunctionReceptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1.
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Tissue specificityExpressed in lymphoid tissues and activated T cells.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 10663 Human
- Omim: 605163 Human
- SwissProt: O00574 Human
- Unigene: 34526 Human
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Alternative names
- bonzo antibody
- C X C chemokine receptor type 6 antibody
- C-X-C chemokine receptor type 6 antibody
see all
Images
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Western blot - Anti-CXCR6 antibody (ab8023)All lanes : Anti-CXCR6 antibody (ab8023) at 1/500 dilution
Lane 1 : human spleen tissue lysate with absence of peptide
Lane 2 : human spleen tissue lysate with presence of peptide
Predicted band size: 44 kDa
Observed band size: 44 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody (ab8023)ab8023 at 20µg/ml staining CXCR6 in human spleen cells by IHC
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Flow Cytometry - Anti-CXCR6 antibody (ab8023)This image is courtesy of an anonymous Abreviewab8023 staining CXCR6 in the Human MCF7 cell line by Flow Cytometry. Samples were incubated with primary antibody (1/50 in PBS) for 20 minutes at 4°C. A FITC-conjugated Pig anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody. The gating strategy was live cells.
Protocols
References
This product has been referenced in:
- Ke C et al. Association between CXCL16/CXCR6 expression and the clinicopathological features of patients with non-small cell lung cancer. Oncol Lett 13:4661-4668 (2017). Read more (PubMed: 28599467) »
- Cui W et al. miR-361-5p inhibits hepatocellular carcinoma cell proliferation and invasion by targeting VEGFA. Biochem Biophys Res Commun 479:901-906 (2016). Read more (PubMed: 27641667) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"