Anti-CXCR6 antibody (ab8023)
- Datasheet
- References (8)
- Protocols
Overview
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Product name
Anti-CXCR6 antibody
See all CXCR6 primary antibodies -
Description
Rabbit polyclonal to CXCR6 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to amino acids 11-25 of Human Bonzo.
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Positive control
- Human spleen tissue lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab8023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| Flow Cyt | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P | Use a concentration of 20 µg/ml. | |
| WB | 1/500. Predicted molecular weight: 44 kDa. |
Target
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Function
Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1. -
Tissue specificity
Expressed in lymphoid tissues and activated T cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 10663 Human
- Omim: 605163 Human
- SwissProt: O00574 Human
- Unigene: 34526 Human
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Alternative names
- bonzo antibody
- C X C chemokine receptor type 6 antibody
- C-X-C chemokine receptor type 6 antibody
see all
Images
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Flow Cytometry - Anti-CXCR6 antibody (ab8023)This image is courtesy of an anonymous Abreviewab8023 staining CXCR6 in the Human MCF7 cell line by Flow Cytometry. Samples were incubated with primary antibody (1/50 in PBS) for 20 minutes at 4°C. A FITC-conjugated Pig anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody. The gating strategy was live cells.
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Western blot - Anti-CXCR6 antibody (ab8023)All lanes : Anti-CXCR6 antibody (ab8023) at 1/500 dilution
Lane 1 : human spleen tissue lysate with absence of peptide
Lane 2 : human spleen tissue lysate with presence of peptide
Predicted band size: 44 kDa
Observed band size: 44 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody (ab8023)ab8023 at 20µg/ml staining CXCR6 in human spleen cells by IHC
Protocols
Datasheets and documents
References
This product has been referenced in:
- Tu GW et al. CXCL16/CXCR6 is involved in LPS-induced acute lung injury via P38 signalling. J Cell Mol Med 23:5380-5389 (2019). Read more (PubMed: 31199046) »
- Li S et al. Oxidative stress drives CD8+ T-cell skin trafficking in patients with vitiligo through CXCL16 upregulation by activating the unfolded protein response in keratinocytes. J Allergy Clin Immunol 140:177-189.e9 (2017). Read more (PubMed: 27826097) »
