Anti-CXorf57 antibody (ab228707)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CXorf57 antibody -
Description
Rabbit polyclonal to CXorf57 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CXorf57 (internal sequence). The exact sequence is proprietary.
Database link: Q6NSI4 -
Positive control
- WB: CXorf57-transfected HEK-293T whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab228707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 98 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 55086 Human
- SwissProt: Q6NSI4 Human
- Unigene: 274267 Human
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Alternative names
- CX057_HUMAN antibody
- CXorf57 antibody
- Uncharacterized protein CXorf57 antibody
Images
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Western blot - Anti-CXorf57 antibody (ab228707)All lanes : Anti-CXorf57 antibody (ab228707) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : CXorf57-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 98 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-CXorf57 antibody (ab228707)SKNSH (Human neuroblastoma cell line) cells stained for CXorf57 protein (green) using ab228707 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
References
ab228707 has not yet been referenced specifically in any publications.
