Anti-CXorf57 antibody (ab228707)
Key features and details
- Rabbit polyclonal to CXorf57
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CXorf57 antibody -
Description
Rabbit polyclonal to CXorf57 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CXorf57 (internal sequence). The exact sequence is proprietary.
Database link: Q6NSI4 -
Positive control
- WB: CXorf57-transfected HEK-293T whole cell extracts.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab228707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 98 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 55086 Human
- SwissProt: Q6NSI4 Human
- Unigene: 274267 Human
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Alternative names
- CX057_HUMAN antibody
- CXorf57 antibody
- Uncharacterized protein CXorf57 antibody
Images
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Western blot - Anti-CXorf57 antibody (ab228707)All lanes : Anti-CXorf57 antibody (ab228707) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : CXorf57-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 98 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-CXorf57 antibody (ab228707)SKNSH (Human neuroblastoma cell line) cells stained for CXorf57 protein (green) using ab228707 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab228707 has not yet been referenced specifically in any publications.
