Overview

  • Product name

  • Description

    Rabbit polyclonal to CXXC5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, 15 aa from near the amino terminus of human CXXC5 (NP_057547).

  • Positive control

    • Human brain tissue lysate.
  • General notes


    Predicted to not cross-react with other CXXC protein family members.

Properties

Applications

Our Abpromise guarantee covers the use of ab106533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 33 kDa. Isoform 2 predicted molecular weight 24 kDa
ICC Use a concentration of 2.5 µg/ml.

Target

  • Function

    May indirectly participate in activation of the NF-kappa-B and MAPK pathways. Acts as a mediator of BMP4-mediated modulation of canonical Wnt signaling activity in neural stem cells.
  • Sequence similarities

    Contains 1 CXXC-type zinc finger.
  • Cellular localization

    Nucleus. Cytoplasm. Colocalizes with DVL1 in large bodies localized just outside the nuclear membrane.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing.
  • Alternative names

    • CF5 antibody
    • CXXC finger 5 protein antibody
    • CXXC finger protein 5 antibody
    • CXXC-type zinc finger protein 5 antibody
    • CXXC5 antibody
    • CXXC5_HUMAN antibody
    • HSPC195 antibody
    • Putative MAPK-activating protein PM08 antibody
    • Putative NF-kappa-B-activating protein 102 antibody
    • retinoid-inducible nuclear factor antibody
    • RINF antibody
    • TCCCIA00297 antibody
    • WID antibody
    • WT1-induced Inhibitor of Dishevelled antibody
    see all

Images

  • Anti-CXXC5 antibody (ab106533) at 1 µg/ml + human brain tissue lysate at 15 µg/ml

    Predicted band size: 33 kDa

  • Immunohistochemistry of CXXC5 in human brain tissue with ab106533 at 2.5 µg/ml.
  • Immunofluorescence of CXXC5 in Human Brain cells using ab106533 at 20 ug/ml.

References

This product has been referenced in:

  • Yasar P  et al. Estradiol-Estrogen Receptor a Mediates the Expression of the CXXC5 Gene through the Estrogen Response Element-Dependent Signaling Pathway. Sci Rep 6:37808 (2016). WB ; Human . Read more (PubMed: 27886276) »
  • Kim HY  et al. CXXC5 is a transcriptional activator of Flk-1 and mediates bone morphogenic protein-induced endothelial cell differentiation and vessel formation. FASEB J 28:615-26 (2014). WB ; Mouse . Read more (PubMed: 24136587) »
See all 2 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

I have placed an order for you, however the antibody is currently out of stock; we are expecting it to be in stock on 17th July and will then be dispatched.

Please quote the order number "1122106" to our customer service team for any order related inquiry.

I hope this new vial will be OK. Please do not hesitate to contact me if you don't receive the product in time or if the product fails again.

Have a good weekend!

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Answer

Thank you very much for having patience!

I have requested the exact immunogen information from our collaborator and is now waiting a reply from them. I will send you the details soon.

Currently, we do not have explanation for 120 kDa band; this could be due to non specificity of antibody or due to lysates. The antibody however should detect a 30-35 kDa band.

The idea for replacement was to try a different lot and then seeing, if the new lot gives same result; if yes, then it will be worth identifying the 120kDa band.

We do not have new lot instock however I have requested the lab for its avialability.

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Question
Answer

Thank you very much for providing the protocol details and images.

The protocol seems fine to me however the following protocol optimizations could;

- At Abcam we use 5% BSA for blocking; I would recommend trying BSA instead of milk.
- Boil the lysates for 10 minutes at 100C.

We are not sure about the identity of 120 kDa band so I am sorry we will not be able to comment on this. The immunogen of this antibody is from the N-terminal region of human CXXC5 (http://www.uniprot.org/uniprot/Q7LFL8; this database actually shows 2 isoforms of CXXC5).

I can offer you a different replacement antibody if you would like to compare the results. Could you let me know how you would like to proceed.

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Question
Answer

Thank you very much for sending the details. I have sent an email to my colleague in lab and requested new QC data for this lot. I will contact you with the explanation soon.

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Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

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