Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] (ab11309)
![Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] (ab11309)](https://www.abcam.com/ps/products/11/ab11309/Images/ab11309-468704-anti-beta-tubulin-cy3-antibody-immunofluorescence-fibroblasts-chicken.jpg "Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] (ab11309)")
Key features and details
- Cy3 ® Mouse monoclonal [TUB 2.1] to beta Tubulin
- Suitable for: ICC/IF
- Reacts with: Chicken
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Isotype: IgG1
Overview
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Product name
Cy3 ® Anti-beta Tubulin antibody [TUB 2.1]
See all beta Tubulin primary antibodies -
Description
Cy3 ® Mouse monoclonal [TUB 2.1] to beta Tubulin -
Host species
Mouse -
Conjugation
Cy3 ®. Ex: 552nm, Em: 565nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Chicken -
Immunogen
Full length protein corresponding to beta Tubulin. Purified rat brain tubulin.
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Epitope
Ab11309 specifically recognizes an epitope in the carboxy-terminal part of beta-tubulin (between amino acids 281-446). -
Positive control
- Cultured chicken fibroblasts.
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General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
The product is prepared by conjugation of Cy3 to purified monoclonal anti-beta-tubulin antibody. The conjugate is purified by gel filtration to remove unbound Cy3 fluorophore. F/P Molar Ratio: (Cy3:Ab) 3 to 9.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
Purity
Proprietary Purification -
Clonality
Monoclonal -
Clone number
TUB 2.1 -
Isotype
IgG1 -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (3) |
1/200.
|
| Notes |
|---|
|
ICC/IF
1/200. |
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
Tissue specificity
Ubiquitously expressed with highest levels in spleen, thymus and immature brain. -
Involvement in disease
Cortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1 -
Sequence similarities
Belongs to the tubulin family. -
Domain
The highly acidic C-terminal region may bind cations such as calcium. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. -
Cellular localization
Cytoplasm, cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 396254 Chicken
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Alternative names
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] (ab11309)
Immunofluorescent analysis of methanol fixed, acetone permeabilized chicken fibroblast cells labelling beta-Tubulin-Cy3 using ab11309 at 1/200 dilution.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab11309 has been referenced in 8 publications.
- Touzelet O et al. The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins. Mol Cell Proteomics 19:793-807 (2020). PubMed: 32075873
- Matamoros AJ et al. Knockdown of Fidgetin Improves Regeneration of Injured Axons by a Microtubule-Based Mechanism. J Neurosci 39:2011-2024 (2019). PubMed: 30647150
- Taborska E et al. Restricted and non-essential redundancy of RNAi and piRNA pathways in mouse oocytes. PLoS Genet 15:e1008261 (2019). PubMed: 31860668
- Vielle NJ et al. The Human Upper Respiratory Tract Epithelium Is Susceptible to Flaviviruses. Front Microbiol 10:811 (2019). PubMed: 31057517
- Maier LJ et al. Unraveling mitotic protein networks by 3D multiplexed epitope drug screening. Mol Syst Biol 14:e8238 (2018). PubMed: 30104419
- Groves HE et al. Characterisation of morphological differences in well-differentiated nasal epithelial cell cultures from preterm and term infants at birth and one-year. PLoS One 13:e0201328 (2018). PubMed: 30517096
- Sato C et al. Tau Kinetics in Neurons and the Human Central Nervous System. Neuron 97:1284-1298.e7 (2018). PubMed: 29566794
- Finkenstaedt-Quinn SA et al. Cytoskeleton dynamics in drug-treated platelets. Anal Bioanal Chem 407:2803-9 (2015). PubMed: 25701419