Cy5 ® Anti-CD19 antibody [6D5] (ab25295)

Submit a review Submit a question
Flow Cytometry - Cy5 ® Anti-CD19 antibody [6D5] (ab25295)

    Key features and details

    • Cy5 ® Rat monoclonal [6D5] to CD19
    • Suitable for: IP, Flow Cyt, Functional Studies
    • Reacts with: Mouse
    • Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    APC PE/Cy5.5® PE/Cy5® PE/Cy7® Unconjugated

    Overview

    • Product name

      Cy5 ® Anti-CD19 antibody [6D5]
      See all CD19 primary antibodies

    • Description

      Cy5 ® Rat monoclonal [6D5] to CD19

    • Host species

      Rat

    • Conjugation

      Cy5 ®. Ex: 650nm, Em: 667nm

    • Tested applications

      Suitable for: IP, Flow Cyt, Functional Studiesmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Tissue, cells or virus corresponding to Mouse CD19. Mouse CD19-expressing K562 human erythroleukemia cells

    • Positive control

      • Flow Cyt: BALB/c mouse splenocytes.

    • General notes

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab25295 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.

    ab136584 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
    Functional Studies
    Use at an assay dependent concentration.
    Notes
    IP
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.

    ab136584 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
    Functional Studies
    Use at an assay dependent concentration.

    Target

    • Function

      Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

    • Involvement in disease

      Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.

    • Sequence similarities

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

    • Post-translational
      modifications

      Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Antibody deficiency due to defect in CD19 antibody
        • Antibody deficiency due to defect in CD19, included antibody
        • AW495831 antibody
        • B lymphocyte antigen CD19 antibody
        • B lymphocyte surface antigen B4 antibody
        • B-lymphocyte antigen CD19 antibody
        • B-lymphocyte surface antigen B4 antibody
        • B4 antibody
        • CD19 antibody
        • CD19 antigen antibody
        • CD19 molecule antibody
        • Cd19 protein antibody
        • CD19_HUMAN antibody
        • CVID3 antibody
        • Differentiation antigen CD19 antibody
        • Leu 12 antibody
        • Leu-12 antibody
        • Leu12 antibody
        • MGC109570 antibody
        • MGC12802 antibody
        • T-cell surface antigen Leu-12 antibody
        see all

      Images

      • Flow Cytometry - Cy5 ® Anti-CD19 antibody [6D5] (ab25295)
        Flow Cytometry - Cy5 ® Anti-CD19 antibody [6D5] (ab25295)

        Flow cytometric analysis of BALB/c mouse splenocytes labeling CD19 using ab25295 at 0.2 µg/106 cells in 100 µl. CD3 is labeled using a Rat Anti-Mouse CD3 epsilon FITC conjugate.

      References (0)

      Publishing research using ab25295? Please let us know so that we can cite the reference in this datasheet.

      ab25295 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab25295.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com