Cy5 ® Anti-CD19 antibody [6D5] (ab25295)
![Flow Cytometry - Cy5 ® Anti-CD19 antibody [6D5] (ab25295)](https://www.abcam.com/ps/products/25/ab25295/Images/ab25295-1-anti-cd19-antibody-6d5-pe-cy5-5-flow-cytometry-splenocytes-balbc-mouse.jpg "Flow Cytometry - Cy5 ® Anti-CD19 antibody [6D5] (ab25295)")
Key features and details
- Cy5 ® Rat monoclonal [6D5] to CD19
- Reacts with: Mouse
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Isotype: IgG2a
Overview
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Product name
Cy5 ® Anti-CD19 antibody [6D5]
See all CD19 primary antibodies -
Description
Cy5 ® Rat monoclonal [6D5] to CD19 -
Host species
Rat -
Conjugation
Cy5 ®. Ex: 650nm, Em: 667nm -
Specificity
ab25295 recognises CD19 B-cell differentiation antigen. -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue/ cell preparation: Mouse CD19 expressing K562 human erythroleukemia cells
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Positive control
- Flow Cyt: BALB/c mouse splenocytes.
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS
Stabilising agent. -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
6D5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
In vitro and in vivo FuncS: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. -
Involvement in disease
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12478 Mouse
- SwissProt: P25918 Mouse
- Unigene: 4360 Mouse
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Alternative names
- Antibody deficiency due to defect in CD19 antibody
- Antibody deficiency due to defect in CD19, included antibody
- AW495831 antibody
see all
Images
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Flow Cytometry - Cy5 ® Anti-CD19 antibody [6D5] (ab25295)
Flow cytometric analysis of BALB/c mouse splenocytes labeling CD19 using ab25295 at 0.2 µg/106 cells in 100 µl. CD3 is labeled using a Rat Anti-Mouse CD3 epsilon FITC conjugate.
Protocols
References (0)
ab25295 has not yet been referenced specifically in any publications.
