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    cy5--cd451-antibody-a20-ab24982.pdf

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Immunology Adaptive Immunity B Cells CD
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Cy5 ® Anti-CD45.1 antibody [A20] (ab24982)

  • Datasheet
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Key features and details

  • Cy5 ® Mouse monoclonal [A20] to CD45.1
  • Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
  • Isotype: IgG2a

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Conjugation
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Cy5® Conjugation Kit (Fast) - Lightning-Link® (ab188288)

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Overview

  • Product name

    Cy5 ® Anti-CD45.1 antibody [A20]
    See all CD45.1 primary antibodies
  • Description

    Cy5 ® Mouse monoclonal [A20] to CD45.1
  • Host species

    Mouse
  • Conjugation

    Cy5 ®. Ex: 650nm, Em: 667nm
  • Specificity

    ab24982 recognizes all mouse leukocytes expressing the CD45.1 allotype.
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Sodium azide
    Constituent: PBS

    Stabilising agent.
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    A20
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Phosphatases

Associated products

  • Alternative Versions

    • PerCP/Cy5.5® Anti-CD45.1 antibody [A20] (ab210237)
    • violetFluor™ 450 Anti-CD45.1 antibody [A20] (ab241939)
    • FITC Anti-CD45.1 antibody [A20] (ab24917)
    • Biotin Anti-CD45.1 antibody [A20] (ab25011)
    • Anti-CD45.1 antibody [A20] (ab25078)
    • APC Anti-CD45.1 antibody [A20] (ab25348)
    • PE/Cy5® Anti-CD45.1 antibody [A20] (ab25349)
    • APC/Cy5.5® Anti-CD45.1 antibody [A20] (ab81989)

Target

  • Relevance

    CD45 is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants and as allelic forms CD45.1/Ly5.1 and CD45.2/Ly5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies in which resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation.
  • Cellular localization

    Type I membrane protein.
  • Alternative names

    • B220 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • LY5 antibody
    • Protein tyrosine phosphatase receptor type C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Receptor type tyrosine protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab24982? Please let us know so that we can cite the reference in this datasheet.

ab24982 has not yet been referenced specifically in any publications.

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