Product nameAnti-Cy5 antibody [CY5-15]
DescriptionMouse monoclonal [CY5-15] to Cy5
Tested applicationsSuitable for: Dot blot, ELISA, IP, ICC, IHC-FoFr, Flow Cytmore details
Other Immunogen Type corresponding to Cy5. A mixture of proteins labelled with Cy5.
- IHC-Free Floating: Rat brain tissue. Flow Cytometry: Purified human CD4+ cells.
Monoclonal antibodies to Cy5 are an important tool for Cy5 signal amplification in assays where biomolecules are labelled with Cy5.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
Purification notesPurified Immunoglobulin
Primary antibody notesMonoclonal antibodies to Cy5 are an important tool for Cy5 signal amplification in assays where biomolecules are labelled with Cy5.
Our Abpromise guarantee covers the use of ab52061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use a concentration of 1 - 2 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 24376610
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceCy3 and Cy5 are reactive watersoluble fluorescent dyes of the cyanine dye family used for labelling proteins, peptides, DNA, RNA, and other biomolecules. They are usually synthesized with reactive groups on either one or both of the nitrogen side chains so that they can be chemically cross-linked to nucleic acids or protein molecules. Thay are small, pH insensitive (between pH 3 and pH 10), soluble in aqueous solution and have tolerance to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. They are used in many different biological assays, including DNA microarrays, protein microarrays, two-dimensional protein analysis, fluorescence resonance energy transfer, and immunocytochemistry. Cy3 is excited maximally at 550 nm and emits maximally at 570 nm, in the green part of the spectrum. Cy5 is excited maximally at 649 nm and emits maximally at 670 nm, in the red part of the spectrum.
αOrai1 internalization in purified human CD4+ cells was measured following incubation at 37°C by flow cytometry using 2 µg/mL αOrai1-AF647 and cell surface detection with biotinylated ab52061 followed by streptavidin-BV421. Samples at 4°C were analyzed in parallel as negative controls for internalization. Data is calculated as percentage of total CD4+ cells and is the average of three donors. D) Representative flow cytometry plots of surface bound αOrai1 compared to total internalized and surface αOrai1. Panel D is representative of two experiments.
ab52061 staining Cy5 in rat brain tissue by Immunohistochemistry (Free Floating sections). Samples were incubated with the primary antibody at a 1/50 dilution for 20 hours at 20°C. A biotin-conjugated horse anti-mouse IgG, rat-adsorbed monoclonal was used as secondary antibody at a 1/200 dilution.
This product has been referenced in:
- Rossiello F et al. DNA damage response inhibition at dysfunctional telomeres by modulation of telomeric DNA damage response RNAs. Nat Commun 8:13980 (2017). Read more (PubMed: 28239143) »
- Bolós M et al. Soluble Tau has devastating effects on the structural plasticity of hippocampal granule neurons. Transl Psychiatry 7:1267 (2017). IHC ; Mouse . Read more (PubMed: 29217824) »