• Product name
    Anti-Cy5 antibody [CY5-15]
  • Description
    Mouse monoclonal [CY5-15] to Cy5
  • Host species
  • Tested applications
    Suitable for: Dot blot, ELISA, IP, ICC, IHC-FoFr, Flow Cytmore details
  • Immunogen

    Other Immunogen Type corresponding to Cy5. A mixture of proteins labelled with Cy5.

  • Positive control
    • IHC-Free Floating: Rat brain tissue. Flow Cytometry: Purified human CD4+ cells.
  • General notes

    Monoclonal antibodies to Cy5 are an important tool for Cy5 signal amplification in assays where biomolecules are labelled with Cy5.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purification notes
    Purified Immunoglobulin
  • Primary antibody notes
    Monoclonal antibodies to Cy5 are an important tool for Cy5 signal amplification in assays where biomolecules are labelled with Cy5.
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab52061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use a concentration of 1 - 2 µg/ml.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-FoFr 1/50.
Flow Cyt Use at an assay dependent concentration. PubMed: 24376610

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Relevance
    Cy3 and Cy5 are reactive watersoluble fluorescent dyes of the cyanine dye family used for labelling proteins, peptides, DNA, RNA, and other biomolecules. They are usually synthesized with reactive groups on either one or both of the nitrogen side chains so that they can be chemically cross-linked to nucleic acids or protein molecules. Thay are small, pH insensitive (between pH 3 and pH 10), soluble in aqueous solution and have tolerance to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. They are used in many different biological assays, including DNA microarrays, protein microarrays, two-dimensional protein analysis, fluorescence resonance energy transfer, and immunocytochemistry. Cy3 is excited maximally at 550 nm and emits maximally at 570 nm, in the green part of the spectrum. Cy5 is excited maximally at 649 nm and emits maximally at 670 nm, in the red part of the spectrum.


  • αOrai1 internalization in purified human CD4+ cells was measured following incubation at 37°C by flow cytometry using 2 µg/mL αOrai1-AF647 and cell surface detection with biotinylated ab52061 followed by streptavidin-BV421. Samples at 4°C were analyzed in parallel as negative controls for internalization. Data is calculated as percentage of total CD4+ cells and is the average of three donors. D) Representative flow cytometry plots of surface bound αOrai1 compared to total internalized and surface αOrai1. Panel D is representative of two experiments.

  • ab52061 staining Cy5 in rat brain tissue by Immunohistochemistry (Free Floating sections). Samples were incubated with the primary antibody at a 1/50 dilution for 20 hours at 20°C. A biotin-conjugated horse anti-mouse IgG, rat-adsorbed monoclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview


This product has been referenced in:
  • Rossiello F  et al. DNA damage response inhibition at dysfunctional telomeres by modulation of telomeric DNA damage response RNAs. Nat Commun 8:13980 (2017). Read more (PubMed: 28239143) »
  • Bolós M  et al. Soluble Tau has devastating effects on the structural plasticity of hippocampal granule neurons. Transl Psychiatry 7:1267 (2017). IHC ; Mouse . Read more (PubMed: 29217824) »

See all 3 Publications for this product

Customer reviews and Q&As

Leider sehe ich ein paar Probleme bei diesem Protokoll:

ab52061 wurde nicht getestet ob er auch Cy5.5 erkennt. Das Immunogen war eine Mischung aus Cy5 konjugierten Proteinen.

Falls ab52061 Cy5.5 erkennen würde, hä...

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Unfortunately, ab52061 has not been tested against either Cy5.5 nor Cy7. Based on the dissimilarity in chemical structure of Cy5.5 and Cy7 vs Cy5, it seems unlikely that ab52061 will recognize either Cy5.5 or Cy7.

Thank you for contacting us.

Unfortunately we do not have any images of product ab52061 and ab52060 with Alexa 647. Alexa 647 is based on the structure of Cy5 and therefore is structurally very similar. As our data sheet states this antibody...

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Thank you for your inquiry.

Unfortunately I am sorry to confirm we have no data or experimental information to verify whether ab52061 Cy5 antibody [CY5-15]cross reacts with Cy5.5. The Cy5 and Cy5.5 structures are quite similar and so the pos...

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Thank you for contacting us.

Unfortunately we have no data or experimental information to verify whether product ab52061 (anti-Cy5) cross reacts with AF-680.

Please do not hesitate to contact us if you need any more advice or info...

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Thank you for your inquiry.

Unfortunately we have no information on whether this antibody cross-reacts with Cy5.5. However looking at a comparison of the molecules that I have attached I believe that it is probably highly unlikely that it wil...

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Thank you and your customer for your inquiry.

I am happy to confirm that ab52061 will only bind to Cy5 and not to the Rhodamine related Tamra or to biotin.

This is tested and guaranteed by our Abpromise.

I wish your custom...

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Immunohistochemistry free floating
Rat Tissue sections (brain)

Mrs. Gitte Toft

Verified customer

Submitted Nov 04 2010


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