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Other Immunogen Type corresponding to Cy5. A mixture of proteins labelled with Cy5.
Monoclonal antibodies to Cy5 are an important tool for Cy5 signal amplification in assays where biomolecules are labelled with Cy5.
Our Abpromise guarantee covers the use of ab52061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use a concentration of 1 - 2 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 24376610
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
αOrai1 internalization in purified human CD4+ cells was measured following incubation at 37°C by flow cytometry using 2 µg/mL αOrai1-AF647 and cell surface detection with biotinylated ab52061 followed by streptavidin-BV421. Samples at 4°C were analyzed in parallel as negative controls for internalization. Data is calculated as percentage of total CD4+ cells and is the average of three donors. D) Representative flow cytometry plots of surface bound αOrai1 compared to total internalized and surface αOrai1. Panel D is representative of two experiments.
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