Product nameCy5.5 Conjugation Kit - Lightning-Link®
This product is manufactured by Expedeon, an Abcam company. Expedeon product code 343-0010 was previously called Lightning-Link® Rapid Cy5.5 Labeling Kit 3 reactions each up to 200 µg and is the same as the 3 x 100 µg size of this product. Expedeon product code 343-0030 was previously called Lightning-Link® Rapid Cy5.5 Labeling Kit 3 reactions each up to 20 µg and is the same as the 3 x 10 µg size of this product. Expedeon product code 343-0005 was previously called Lightning-Link® Rapid Cy5.5 Labeling Kit 1 reaction up to 200 µg and is the same as the 100 µg size of this product.
Cy5.5 Conjugation Kit / Cy5.5 Labeling Kit ab269891 uses a simple and quick process for Cy5.5 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.
To conjugate an antibody to Cy5.5 using this kit:
- add modifier to antibody and incubate for 15 mins
- add quencher and incubate for 5 mins
The Cy5.5 conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to Cy5.5.
Amount and volume of antibody for conjugation to Cy5.5
Kit size Recommended
amount of antibody1
amount of antibody
30 µg 3 x 10 µg 3 x 20 µg 3 x 10 µL 100 µg 100 µg 200 µg 100 µL 300 µg 3 x 100 µg 3 x 200 µg 3 x 100 µL
1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
50mM / 0.6% Tris1 0.1% BSA2 50% glycerol 0.1% sodium azide PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
Thiomerosal Proclin Glycine Arginine Glutathione DTT
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 µg 3 x 10 µg 3 x 100 µg 30 µg Cy5.5 mix (Lyophilized) 1 vial 3 vials 3 vials 3 vials Modifier reagent 1 vial 1 vial 1 vial 1 vial Quencher reagent 1 vial 1 vial 1 vial 1 vial
ab269891 has not yet been referenced specifically in any publications.