For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Are fluorescent conjugation kits (Cy5 labeling kit) suitable for the labeling of M13 bacteriophage through amine group?
Asked on Jan 15 2015
M13 bacteriophages have not been tested with our labeling kits. However, as long as it contains some primary amines which are available for labeling, then there should be no reason why this would not work. As for antibodies, it is important that the buffer does not contain any free amines or other unsuitable substances. Please see below for details of (un)compatible formulations:
Additives such as salts (e.g NaCl), sugars (e.g. sucrose) and chelators (e.g. EDTA) have no effect on the labeling reaction. We recommend avoiding nucleophiles such as amino acids (e.g. glycine), blockers (e.g. ethanolamine) and thiols (DTT, mercaptoethanol) that could interfere with the conjugation reaction.
up to 20mM Tris
up to 0.1% BSA (BSA up-to 1% will work but yield lower quality conjugates)
up to 0.1% gelatin
up to 0.1% sodium azide
up to 50% glycerol
0.15M sodium chloride
0.02M potassium phosphate
Tris above 20mM
Finally, the M13 bacteriophage will be a different molecular weight to that of an antibody, and the protocol will therefore have to be adjusted to allow for the size difference. It is difficult for us to provide any advice, as I do not know the MW of the bacteriophage, but I would generally recommend that you carry out a titration of different concentrations, in order to determine the best set-up for your assay.
Answered on Jan 15 2015