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I have recently purchased (ab188288 – Cy5 Fast Conjugation Kit) to label an Antibody for a flowcytometry. But the storage buffer of the antibody contains 1XPBS, 1%BSA, 20% Glycerol (pH7). And 0.01% Thimerosal was added as a preservative, would this has an effect on the conjugation. Should I do dialysis for the antibody before labelling?
Asked on Mar 18 2015
Only the BSA seems to be a problem, its concentration should be 0.1%-0.5%. The ideal way is to remove BSA, we have kit for this https://www.abcam.com/BSA-Removal-Kit-ab173231.html. Alternatively you can give it a go however it might affect the efficiency of the kit. Please check the information on booklet;
10.2 Common non-buffering salts (e.g. sodium chloride), chelating agents (e.g.EDTA), and sugars have no effect on conjugation efficiency. Azide (0.02 to 0.1%) and BSA (0.1 to 0.5%) have little or no effect. Glycerol up to 50% has no effect.
Padamjeet SinghAbcam Scientific Support
Answered on Mar 18 2015