Biotin Anti-Cyanine antibody (ab7625)
Key features and details
- Biotin Sheep polyclonal to Cyanine
- Suitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Cyanine antibody
See all Cyanine primary antibodies -
Description
Biotin Sheep polyclonal to Cyanine -
Host species
Sheep -
Conjugation
Biotin -
Tested applications
Suitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Cyanine conjugated to keyhole limpet haemocyanin. A mixture of Cy3 and Cy5 conjugated KLH
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General notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Sheep IgG molecule
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
Dot blot | ||
ELISA | ||
IHC-P | ||
IHC-Fr | ||
Immunomicroscopy |
Notes |
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ELISA: 1/8000 - 1/40000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab7625 has not yet been referenced specifically in any publications.