Key features and details
- Rabbit polyclonal to CYB561A3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CYB561A3 antibody
DescriptionRabbit polyclonal to CYB561A3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- IHC-P: Human stomach tissue; WB: Transfected HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 27 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFerric-chelate reductase that reduces Fe(3+) to Fe(2+) before its transport from the endosome to the cytoplasm. Probably uses ascorbate as electron donor.
Sequence similaritiesContains 1 cytochrome b561 domain.
Cellular localizationLate endosome membrane. Lysosome membrane.
- Information by UniProt
- CYAC3_HUMAN antibody
- Cyb561a3 antibody
- CYBASC3 antibody
All lanes : Anti-CYB561A3 antibody (ab185304) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : CYB561A3 over-expression - HEK-293T whole cell lysate, (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Predicted band size: 27 kDa
Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling CYB561A3 in the membrane and cytoplasm of glandular cells with ab185304 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab185304 has not yet been referenced specifically in any publications.