Key features and details
- Rabbit polyclonal to CYB5B
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CYB5B antibody
See all CYB5B primary antibodies
DescriptionRabbit polyclonal to CYB5B
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
- WB: HepG2, U-87 MG, EC109 , HeLa and A549 whole cell lysates; mouse heart and liver lysates. IHC-P: Human kidney tissue. ICC/IF: HepG2 cells.
This product was previously labelled as Cytochrome b5 Outer Mitochondrial Membrane
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 16 kDa.|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
RelevanceCytochrome b5 Outer Mitochondrial Membrane belongs to the cytochrome b5 family and contains one cytochrome b5 heme-binding domain. Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Cellular localizationMitochondrion outer membrane
- CYB5 M antibody
- CYB5M antibody
- Cytochrome b5 type B antibody
All lanes : Anti-CYB5B antibody (ab236612) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse heart lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 6 : EC109 whole cell lysate
Lane 7 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 16 kDa
Paraffin-embedded human kidney tissue stained for CYB5B using ab236612 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CYB5B (green) using ab236612 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236612 has not yet been referenced specifically in any publications.