• Product name

    Anti-CYB5R3 antibody [EPR3868]
    See all CYB5R3 primary antibodies
  • Description

    Rabbit monoclonal [EPR3868] to CYB5R3
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in human CYB5R3 (P00387).

  • Positive control

    • A431; Fetal liver; HeLa and U87 MG lysates. human testis tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab133247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
    • Tissue specificity

      Isoform 2 is expressed at late stages of erythroid maturation.
    • Involvement in disease

      Defects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3) [MIM:250800]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well-tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms.
    • Sequence similarities

      Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
      Contains 1 FAD-binding FR-type domain.
    • Cellular localization

      Endoplasmic reticulum membrane. Mitochondrion outer membrane and Cytoplasm. Produces the soluble form found in erythrocytes.
    • Information by UniProt
    • Database links

    • Alternative names

      • B5R antibody
      • Cyb5r3 antibody
      • Cytochrome b5 reductase 3 antibody
      • Cytochrome b5 reductase antibody
      • DIA1 antibody
      • Diaphorase 1 antibody
      • Diaphorase-1 antibody
      • NADH cytochrome b5 reductase 3 antibody
      • NADH-cytochrome b5 reductase 3 membrane-bound form antibody
      • NADH-cytochrome b5 reductase 3 soluble form antibody
      • NB5R3_HUMAN antibody
      • OTTHUMP00000028761 antibody
      • OTTHUMP00000198435 antibody
      • OTTHUMP00000198574 antibody
      • OTTHUMP00000198662 antibody
      • OTTHUMP00000198665 antibody
      see all


    • All lanes : Anti-CYB5R3 antibody [EPR3868] (ab133247) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Fetal liver cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : U87 MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 34 kDa

      Note: Primary antibody ab133247 is diluted with 1% BSA
    • Immunohistochemical analysis of paraffin embedded Human testis tissue labelling CYB5R3 with ab133247 at 1/50 dilution.


    ab133247 has not yet been referenced specifically in any publications.

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