Anti-CYC1 antibody (ab224044)
Key features and details
- Rabbit polyclonal to CYC1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CYC1 antibody
See all CYC1 primary antibodies -
Description
Rabbit polyclonal to CYC1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human CYC1 aa 150 to the C-terminus.
Database link: P08574 -
Positive control
- IHC: Human stomach tissue. WB: MCF7, NIH/3T3 and NBT-II cell lysate. ICC: U-251 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain. -
Sequence similarities
Belongs to the cytochrome c family.
Contains 1 cytochrome c domain. -
Post-translational
modificationsBinds 1 heme group per subunit. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 512500 Cow
- Entrez Gene: 1537 Human
- Omim: 123980 Human
- SwissProt: P00125 Cow
- SwissProt: P08574 Human
- Unigene: 289271 Human
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Alternative names
- Complex III subunit 4 antibody
- Complex III subunit IV antibody
- CY1_HUMAN antibody
see all
Images
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Immunohistochemistry analysis of human skeletal tissue labelling CYC1 with ab224044 at 1/200.
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Immunohistochemistry analysis of human kidney tissue labelling CYC1 with ab224044 at 1/200.
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Immunohistochemistry analysis of human duodenum tissue labelling CYC1 with ab224044 at 1/200.
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Immunohistochemistry analysis of human cerebral cortex tissue labelling CYC1 with ab224044 at 1/200.
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Anti-CYC1 antibody (ab224044) at 1/100 dilution + MCF7 (human breast adenocarcinoma cell line) cell lysate
Predicted band size: 35 kDa -
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for CYC1 (green) using ab224044 at 4 µg/ml in ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab224044 has been referenced in 1 publication.
- Lu T et al. Long non-coding RNA C5orf66-AS1 prevents oral squamous cell carcinoma through inhibiting cell growth and metastasis. Int J Mol Med 42:3291-3299 (2018). PubMed: 30280186