Cyclic AMP Direct ELISA Kit (ab133038)

The “regular” cAMP kit, Cyclic AMP ELISA Kit (ab133039) comes with an assay buffer for diluting standards and samples, and is intended for use with extracellular cAMP – samples like serum or cell culture media, for example.


In the “direct” cAMP kit., Cyclic AMP Direct ELISA Kit (ab133038), the assay buffer has been replaced with 0.1M HCl and a neutralizing reagent has been included. Samples such as cell culture cells or tissue can be lysed in that HCl, then loaded directly into the wells (to which the neutralizing reagent has been added), hence the “direct” in the name.


The “complete” kit, Cyclic AMP Complete ELISA Kit (ab133051) simply includes both the assay buffer from the regular kit and the HCl/ neutralizing reagent combo from the direct kit so you can measure samples from anything. Please note that all the other components of the kit – plate, antibody, conjugate, standard, etc – are identical in all three cases.


As you would like to measure cell lysates and supernatant samples and therefore I would recommend kit ab133051.


Please note that the sensitivity is always higher when the samples is acetylated.

Thank you for your enquiry.
I appreciate your patience, I have been discussing this question with the laboratories.

Actually all three cAMP kits are essentially the exact same kit; all kit components are identical with the exception of the buffers/materials provided to process the samples.

The ab133038 kit is intended for intracellular cAMP detection and comes with 0.1MHCl for lysis and sample/standard dilution as well as a neutralization reagent to balance the pH.
The ab133039 kit is intended for extracellular cAMP detection and comes with assay buffer for sample/standard dilution.
The complete cAMP kit, ab133039, simply comes with 0.1MHCl, neutralization reagent, and assay buffer so that researchers can look at both intracellular and extracellular cAMP.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

Gracias por contactarnos de nuevo, y por tu soporte a nuestros productos. Siento la tardanza en contestar.

Respecto a las preguntas del kit ab133038:

1) La fecha de caducidad del producto varía de lote a lote. Nosotros garantizamos su funcionamiento en los 6 meses siguientes a la fecha de compra, y siempre recomendamos comprar el kit cuando vaya a utilizarse, en lugar de dejarlo almacenado y usarlo al cabo de los meses.

2) Respecto a las publicaciones, me temo que si en la datasheet del producto no hay ninguna indicada, es porque lamentablemente no tenemos constancia de ninguna referencia en la que se haya usado este kit. Tan pronto como conozcamos de la existencia de alguna referencia trabajando con este kit, actualizaremos la página web.

3) El kit está pensado para la detección intracelular de cAMP. Siempre que haya cAMP presente en las muestras, el kit debiera detectarlo. No tenemos experiencia en el uso del kit en células de osteosarcoma de rata, pero recomendaría a la clienta que buscara literatura para conocer los niveles de cAMP esperados en las muestras de interés.

Espero que esta información sea útil para la clienta. En caso contrario, no dudes en comentármelo, y estaré encantada de poder ayudar.

Thank you for your enquiry.

The kit is provided with break apart strips which can be used only once.

Just let me know if there is any more information you need.