Product nameCyclic AMP ELISA Kit
See all cAMP kits
Sample typeCell culture supernatant, Saliva, Serum
Assay time3h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human, a wide range of other species
Does not react with: Rabbit
Abcam’s Cyclic AMP in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) is a colorimetric competitive enzyme immunoassay kit for the quantitative determination of extracellular cAMP.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-cAMP antigen and a polyclonal rabbit antibody specific to cAMP. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of cAMP captured in the plate.
Please note: This kit is not suitable for samples of rabbit serum and plasma since the endogenous IgG will interfere with the assay.
Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.
Compound % Cross Reactivity cAMP 100 AMP <0.33 ATP <0.12 cGMP <0.001 GMP <0.001 GTP <0.001 cUMP <0.001 CTP <0.001
Tested applicationsSuitable for: Competitive ELISAmore details
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Acetylation kit - Acetic Anhydride 1 x 1ml Acetylation kit - Triethylamine 1 x 2ml Assay Buffer 2 1 x 27ml Cyclic AMP Alkaline Phosphatase Conjugate 1 x 5ml Cyclic AMP Complete Antibody 1 x 5ml Cyclic AMP Complete Standard 1 x 500µl Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml
Relevancedblinks not applicable
- 3'-5'-cyclic adenosine monophosphate
- Cyclic adenosine monophosphate
- cyclic AMP
Our Abpromise guarantee covers the use of ab133039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
ab133039 has not yet been referenced specifically in any publications.