Overview

  • Product name

    Cyclic AMP ELISA Kit
    See all cAMP kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Saliva, Serum
  • Assay type

    Competitive
  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human, a wide range of other species
    Does not react with: Rabbit
  • Product overview

    Abcam’s Cyclic AMP in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) is a colorimetric competitive enzyme immunoassay kit for the quantitative determination of extracellular cAMP.


    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-cAMP antigen and a polyclonal rabbit antibody specific to cAMP. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of cAMP captured in the plate.


    Please note: This kit is not suitable for samples of rabbit serum and plasma since the endogenous IgG will interfere with the assay.

     

  • Notes

    Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.

    Cross Reactivity

    Compound % Cross Reactivity
    cAMP 100
    AMP <0.33
    ATP <0.12
    cGMP <0.001
    GMP <0.001
    GTP <0.001
    cUMP <0.001
    CTP <0.001
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 27ml
    Acetylation kit - Acetic Anhydride 1 x 1ml
    Acetylation kit - Triethylamine 1 x 2ml
    Assay Buffer 2 1 x 27ml
    Cyclic AMP Alkaline Phosphatase Conjugate 1 x 5ml
    Cyclic AMP Complete Antibody 1 x 5ml
    Cyclic AMP Complete Standard 1 x 500µl
    Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit
    Plate Sealer 1 unit
    pNpp Substrate 1 x 20ml
    Stop Solution 1 x 5ml
  • Research areas

  • Relevance

    dblinks not applicable
  • Alternative names

    • 3'-5'-cyclic adenosine monophosphate
    • Cyclic adenosine monophosphate
    • cyclic AMP

Applications

Our Abpromise guarantee covers the use of ab133039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133039.

  • Representative Standard Curve using ab133039.

Protocols

References

ab133039 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer




The “regular” cAMP kit, Cyclic AMP ELISA Kit (ab133039) comes with an assay buffer for diluting standards and samples, and is intended for use with extracellular cAMP – samples like serum or cell culture media, for example.


In the “direct” cAMP kit., Cyclic AMP Direct ELISA Kit (ab133038), the assay buffer has been replaced with 0.1M HCl and a neutralizing reagent has been included. Samples such as cell culture cells or tissue can be lysed in that HCl, then loaded directly into the wells (to which the neutralizing reagent has been added), hence the “direct” in the name.


The “complete” kit, Cyclic AMP Complete ELISA Kit (ab133051) simply includes both the assay buffer from the regular kit and the HCl/ neutralizing reagent combo from the direct kit so you can measure samples from anything. Please note that all the other components of the kit – plate, antibody, conjugate, standard, etc – are identical in all three cases.


As you would like to measure cell lysates and supernatant samples and therefore I would recommend kit ab133051.


Please note that the sensitivity is always higher when the samples is acetylated.


Read More

Answer

Thank you for your enquiry.
I appreciate your patience, I have been discussing this question with the laboratories.

Actually all three cAMP kits are essentially the exact same kit; all kit components are identical with the exception of the buffers/materials provided to process the samples.

The ab133038 kit is intended for intracellular cAMP detection and comes with 0.1MHCl for lysis and sample/standard dilution as well as a neutralization reagent to balance the pH.
The ab133039 kit is intended for extracellular cAMP detection and comes with assay buffer for sample/standard dilution.
The complete cAMP kit, ab133039, simply comes with 0.1MHCl, neutralization reagent, and assay buffer so that researchers can look at both intracellular and extracellular cAMP.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

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Answer

Thank you for contacting us. Yes, this Cyclic AMP ELISA Kit will be guaranteed for use on mouse samples.

Read More

Answer

Thank you for contacting us.

This kit can be used to detect cAMP level in serum, saliva, urine or culture supernatants. Theoretically this kit will provide information about the activity of PTH hormone as it is involved in generation of cAMP. We however haven't tried this in similar kind of experiment so I am sorry we will not be able to provide any tested data. You might need to design your own experiment with this kit.

Please also check the PTH activity related publications in PubMed these will provide you further insight in your research.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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