Overview

  • Product name

    Cyclic GMP Complete ELISA Kit
    See all cGMP kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Plasma, Tissue, Cell Lysate
  • Assay type

    Competitive
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 102.9 % - %
    Urine 97.7 % - %
    Serum 101.3 % - %
    Tissue Culture Media 101.7 % - %
    Heparin Plasma 104.4 % - %
    EDTA Plasma 115 % - %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Product overview

    Abcam’s Cyclic GMP Complete in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cyclic GMP Complete in cells and tissue treated with 0.1N HCl, in addition to culture supernatants, saliva, and serum.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-cGMP antigen and a polyclonal rabbit antibody specific to cGMP. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of cGMP captured in the plate.

  • Notes

    Guanosine 3’,5’-cyclic monophosphate (cyclic GMP; cGMP) was identified in 1963. It has been shown to be present at levels typically.10-100 fold lower than cAMP in most tissues and is formed by the action of the enzyme.guanylate cyclase on GTP. It is involved in a number of important biological reactions. Some hormones, such as acetylcholine, insulin, and oxytocin, as well as certain other chemicals like serotonin and histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators niroprusside, nitroglycerin, sodium nitrate, and nitric oxide.(NO) also stimulate cGMP levels. Peptides, such as atrial natriuretic peptide (ANP) that relax smooth muscle also increase cGMP concentrations. cGMP has been confirmed as a second messenger for ANP. NO can be synthesized from L-arginine and diffuse through cell membranes. The interaction of NO with guanylate cyclase allows cGMP to act as a third messenger in some cells.

     

    Cross Reactivities:

     

    CompoundCross Reactivity
    cGMP100%
    GMP<0.001%
    GTP<0.001%
    cAMP<0.001%
    AMP<0.001%
    ATP<0.001%
    cUMP<0.001%
    CTP<0.001%
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    0.1M HCl 1 x 27ml
    20X Wash Buffer Concentrate 1 x 27ml
    Acetylation kit - Acetic Anhydride 1 x 1ml
    Acetylation kit - Triethylamine 1 x 2ml
    Assay Buffer 2 1 x 27ml
    Cyclic GMP Complete Alkaline Phosphatase Conjugate 1 x 5ml
    Cyclic GMP Complete Antibody 1 x 5ml
    Cyclic GMP Complete Standard 1 x 500µl
    Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit
    Neutralizing Reagent 1 x 5ml
    Plate Sealer 1 unit
    pNpp Substrate 1 x 20ml
    Stop Solution 1 x 5ml
  • Research areas

  • Relevance

    Cyclic guanosine monophosphate (cGMP) serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG).
  • Alternative names

    • Cyclic GMP
    • Cyclic guanosine monophosphate
    • Guanosine 3 5 Cyclic Monophosphate

Applications

Our Abpromise guarantee covers the use of ab133052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133052. Non-Acetylated Format.

  • Representative Standard Curve using ab133052. Acetylated Format.

Protocols

References

This product has been referenced in:

  • Schaffner D  et al. Analysis of the nitric oxide-cyclic guanosine monophosphate pathway in experimental liver cirrhosis suggests phosphodiesterase-5 as potential target to treat portal hypertension. World J Gastroenterol 24:4356-4368 (2018). Read more (PubMed: 30344420) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
We used this kit to measure levels of cGMP in bone marrow derived macrophages(BMDMs) from C57BL/6 mouse stimulated with different compounds. Sample collection of the cell lysates was done in 0.1M HCl and samples were kept at -20ºC until use ( 2 weeks later). We used the acetylated format due to the low concentration of cGMP expected in the samples and the procedure of the kit was really easy.
We can confirm with our data that the kit works perfectly in mouse samples and specifically in cultures of BMDMs.

Abcam user community

Verified customer

Submitted Jul 22 2015

Cyclic GMP levels in different mouse brain tissues

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We are comparing different mouse models of Huntington's Chorea and evaluating cyclic GMP levels in these mice as compared to wild type animals.

In preparation for this study we looked at several manufacturers Cyclic GMP kits and found that the Abcam kit was the best choice as far as simplicity and reproducibility and have used this kit in this particular study. Preparation of the tissue samples for use in the kit was very easy and results was consistent against different experiments and kits.

Abcam user community

Verified customer

Submitted Jan 19 2015

Answer

Thank you for contacting us.

Both the Direct cGMP ELISA Kit (ab133027) and the cGMP Complete ELISA Kit (ab133052) can be used to detect cGMP in tissue and cell lysates.

Theab133052 kit is a Complete cGMP kit while the ab133027 kit is the Direct cGMP. We also have the cGMP Elisa Kit (ab133026). The ab133027 Direct kit is intended for intracellular cGMP detection and comes with HCl for lysis and dilution of your samples along with a neutralization buffer. The ab133026 kit comes with assay buffer for dilution of standards and samples because this kit is intended for extracellular cGMP detection.The Complete kit, ab133052 comes with HCl, Neutralization buffer, and assay buffer so that you may look at both intracellular and extracellular cGMP.

I hope this has answered your questions, if you have any further questions, please do not hesitate to ask.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up