Key features and details
- Sample type: Cell Lysate, Tissue
- Detection method: Colorimetric
- Assay type: Competitive
Product nameCyclic GMP Direct ELISA Kit
See all cGMP kits
Sample typeTissue, Cell Lysate
Assay durationMultiple steps standard assay
Abcam’s Cyclic GMP (cGMP) Direct in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cyclic GMP in tissue culture media treated with 0.1 M HCl.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-cGMP antigen and a polyclonal rabbit antibody specific to cGMP. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of cGMP captured in the plate.
Guanosine 3’, 5’-cyclic monophosphate (cyclic GMP; cGMP) has been shown to be present at levels typically 10-100 fold lower than cAMP in most tissues and is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones, such as acetylcholine, insulin and oxytocin, as well as certain other chemicals like serotonin and histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate and nitric oxide (NO) also increase cGMP levels. NO can be synthesized from L-arginine and diffuse through cell membranes. The interaction of NO with guanylate cyclase allows cGMP to act as a third messenger in some cells. Bioactive peptides like atrial natriuretic peptide (ANP) also increase cGMP concentrations.
Compound % Cross Reactivity cGMP 100 GMP <0.001 GTP <0.001 cAMP <0.001 AMP <0.001 ATP <0.001 cUMP <0.001 CTP <0.001
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 0.1M HCl 1 x 27ml 20X Wash Buffer Concentrate 1 x 27ml Acetylation kit - Acetic Anhydride 1 x 1ml Acetylation kit - Triethylamine 1 x 2ml Cyclic GMP Complete Antibody 1 x 5ml Cyclic GMP Complete Standard 1 x 500µl Cyclic GMP Direct Alkaline Phosphatase Conjugate 1 x 5ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Neutralizing Reagent 1 x 5ml Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml
RelevanceCyclic guanosine monophosphate (cGMP) serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG).
- Cyclic GMP
- Cyclic guanosine monophosphate
- Guanosine 3 5 Cyclic Monophosphate
ab133027 has been referenced in 1 publication.
- Banerjee J et al. Biphasic Effect of Sildenafil on Energy Sensing is Mediated by Phosphodiesterases 2 and 3 in Adipocytes and Hepatocytes. Int J Mol Sci 20:N/A (2019). PubMed: 31248114