Overview

  • Product name

    Cyclic GMP ELISA Kit
    See all cGMP kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Plasma
  • Assay type

    Competitive
  • Assay time

    3h 0m
  • Assay duration

    Multiple steps standard assay
  • Product overview

    Abcam’s Cyclic GMP (cGMP) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cyclic GMP in saliva, plasma (Heparin, EDTA), urine, and serum and tissue culture supernatants.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-cGMP antigen and a polyclonal rabbit antibody specific to cGMP. After incubation the excess reagents are washed away. pNpp substrate is added. After a short incubation the alkaline phosphatase enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of cGMP captured in the plate.

  • Notes

    Guanosine 3’, 5’-cyclic monophosphate (cyclic GMP; cGMP) has been shown to be present at levels typically 10-100 fold lower than cAMP in most tissues and is formed by the action of the enzyme guanylate cyclase on GTP. It is involved in a number of important biological reactions. Some hormones, such as acetylcholine, insulin and oxytocin, as well as certain other chemicals like serotonin and histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate and nitric oxide (NO) also stimulate cGMP levels. Peptides, such as atrial natriuretic peptide (ANP) that relax smooth muscle also increase cGMP concentrations. cGMP has been confirmed as a second messenger for ANP. NO can be synthesized from L-arginine and diffuse through cell membranes. The interaction of NO with guanylate cyclase allows cGMP to act as a third messenger in some cells.

    Cross Reactivity 

    Compound% Cross Reactivity
    cGMP100
    GMP<0.001
    GTP<0.001
    cAMP<0.001
    AMP<0.001
    ATP<0.001
    cUMP<0.001
    CTP<0.001
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 27ml
    Acetylation kit - Acetic Anhydride 1 x 1ml
    Acetylation kit - Triethylamine 1 x 2ml
    Assay Buffer 2 1 x 27ml
    Cyclic GMP Complete Alkaline Phosphatase Conjugate 1 x 5ml
    Cyclic GMP Complete Antibody 1 x 5ml
    Cyclic GMP Complete Standard 1 x 500µl
    Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit
    Plate Sealer 1 unit
    pNpp Substrate 1 x 20ml
    Stop Solution 1 x 5ml
  • Research areas

  • Relevance

    Cyclic guanosine monophosphate (cGMP) serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG).
  • Alternative names

    • Cyclic GMP
    • Cyclic guanosine monophosphate
    • Guanosine 3 5 Cyclic Monophosphate

Applications

Our Abpromise guarantee covers the use of ab133026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133026.

  • Representative Standard Curve using ab133026.

Protocols

References

ab133026 has not yet been referenced specifically in any publications.

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