Recombinant
RabMAb

Recombinant Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)

Overview

  • Product name

    Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054]
    See all Cyclin A1 + Cyclin A2 primary antibodies
  • Description

    Rabbit monoclonal [EPR18054] to Cyclin A1 + Cyclin A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Cyclin A1 + Cyclin A2 aa 250-450. The exact sequence is proprietary. Immunogen is from Cyclin A1. Cyclin A2: P20248
    Database link: P78396

  • Positive control

    • WB: Recombinant fragment of Human Cyclin A1 protein; Full length Human Cyclin A2 recombinant protein; Human testis lysate. HeLa and HepG2 whole cell lysate. IHC-P: Human testis and colon cancer tissues. IP: K562 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18054
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab185619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 52, 49 kDa (predicted molecular weight: 52, 49 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/70.

Target

  • Relevance

    Cyclin A1: Function: May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Tissue specificity: Very high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines. Similarity: Belongs to the cyclin family. Cyclin AB subfamily. Developmental stage: Expression increases in early G1 phase and reaches highest levels during the S and G2/M phases. Cyclin A2: Function: Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. Similarity: Belongs to the cyclin family. Cyclin AB subfamily. Developmental stage: Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • CCN1 antibody
    • CCNA antibody
    • CCNA1 antibody
    • CCNA2 antibody
    • CT146 antibody
    • Cyclin-A antibody
    • Cyclin-A1 antibody
    • Cyclin-A2 antibody
    see all

Images

  • Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/20000 dilution + Recombinant fragment of Human Cyclin A1 protein at 0.01 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 52, 49 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 seconds


    Recombinant fragment of Human Cyclin A1 protein contains aa261-450 with His-Tag & GST-tag.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Cyclin A1 + Cyclin A2 using ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining of spermatocytes of the human testis is observed. Counterstained with Hematoxylin.
    Negative control obtained using PBS instead of ab185619 and secondary antibody only.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/2000 dilution + Full length Human Cyclin A2 recombinant protein at 0.01 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 52, 49 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Recombinant full length Cyclin A2 protein contains aa1-432 with His-Tag.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer, labeling Cyclin A1 + Cyclin A2 with ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining of human colonic adenocarcinoma cells is observed. Counterstained with Hematoxylin.
    Negative control obtained using PBS instead of ab185619 and secondary antibody only.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 52, 49 kDa
    Observed band size: 49,52 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    5% NFDM/TBST: Blocking and diluting buffer.

  • Cyclin A1 + Cyclin A2 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab185619 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab185619 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: K562 whole cell extract 10 µg (Input).

    Lane 2: ab185619 IP in K562 whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185619 in K562 whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds

  • All lanes : Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 52, 49 kDa
    Observed band size: 49,52 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

References

This product has been referenced in:

  • Qiao E  et al. Long noncoding RNA TALNEC2 plays an oncogenic role in breast cancer by binding to EZH2 to target p57KIP2 and involving in p-p38 MAPK and NF-?B pathways. J Cell Biochem 120:3978-3988 (2019). Read more (PubMed: 30378143) »
  • Tseng YH  et al. Thyroid hormone suppresses expression of stathmin and associated tumor growth in hepatocellular carcinoma. Sci Rep 6:38756 (2016). Human . Read more (PubMed: 27934948) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 07 2015

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