Recombinant Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
![Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)](https://www.abcam.com/ps/products/185/ab185619/Images/ab185619-241616-anti-cyclin-a1-cyclin-a2-antibody-epr18054-western-blot.jpg "Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18054] to Cyclin A1 + Cyclin A2
- Suitable for: WB, IHC-P, IP
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
-
Product name
Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054]
See all Cyclin A1 + Cyclin A2 primary antibodies -
Description
Rabbit monoclonal [EPR18054] to Cyclin A1 + Cyclin A2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Recombinant fragment of Human Cyclin A1 protein; Full length Human Cyclin A2 recombinant protein; Human testis lysate. HeLa and HepG2 whole cell lysate. IHC-P: Human testis and colon cancer tissues. IP: K562 whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18054 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 52, 49 kDa (predicted molecular weight: 52, 49 kDa).
|
|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| IP |
1/70.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 52, 49 kDa (predicted molecular weight: 52, 49 kDa). |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/70. |
Target
-
Relevance
Cyclin A1: Function: May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Tissue specificity: Very high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines. Similarity: Belongs to the cyclin family. Cyclin AB subfamily. Developmental stage: Expression increases in early G1 phase and reaches highest levels during the S and G2/M phases. Cyclin A2: Function: Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. Similarity: Belongs to the cyclin family. Cyclin AB subfamily. Developmental stage: Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 890 Human
- Entrez Gene: 8900 Human
- Omim: 123835 Human
- Omim: 604036 Human
- SwissProt: P20248 Human
- SwissProt: P78396 Human
-
Alternative names
- CCN1 antibody
- CCNA antibody
- CCNA1 antibody
see all
Images
-
Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/20000 dilution + Recombinant fragment of Human Cyclin A1 protein at 0.01 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 52, 49 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsRecombinant fragment of Human Cyclin A1 protein contains aa261-450 with His-Tag & GST-tag.
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)](https://www.abcam.com/ps/products/185/ab185619/Images/ab185619-241612-anti-cyclin-a1-cyclin-a2-antibody-epr18054-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Cyclin A1 + Cyclin A2 using ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining of spermatocytes of the human testis is observed. Counterstained with Hematoxylin.
Negative control obtained using PBS instead of ab185619 and secondary antibody only.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)](https://www.abcam.com/ps/products/185/ab185619/Images/ab185619-241615-anti-cyclin-a1-cyclin-a2-antibody-epr18054-western-blot.jpg)
Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/2000 dilution + Full length Human Cyclin A2 recombinant protein at 0.01 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 52, 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsRecombinant full length Cyclin A2 protein contains aa1-432 with His-Tag.
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)](https://www.abcam.com/ps/products/185/ab185619/Images/ab185619-241611-anti-cyclin-a1-cyclin-a2-antibody-epr18054-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)Immunohistochemical analysis of paraffin-embedded human colon cancer, labeling Cyclin A1 + Cyclin A2 with ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining of human colonic adenocarcinoma cells is observed. Counterstained with Hematoxylin.
Negative control obtained using PBS instead of ab185619 and secondary antibody only.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)](https://www.abcam.com/ps/products/185/ab185619/Images/ab185619-241614-anti-cyclin-a1-cyclin-a2-antibody-epr18054-western-blot.jpg)
Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilution + Human testis lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 52, 49 kDa
Observed band size: 49,52 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute5% NFDM/TBST: Blocking and diluting buffer.
-
![Immunoprecipitation - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)](https://www.abcam.com/ps/products/185/ab185619/Images/ab185619-241610-anti-cyclin-a1-cyclin-a2-antibody-epr18054-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)Cyclin A1 + Cyclin A2 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab185619 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab185619 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: K562 whole cell extract 10 µg (Input).
Lane 2: ab185619 IP in K562 whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185619 in K562 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 5 seconds
-
![Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)](https://www.abcam.com/ps/products/185/ab185619/Images/ab185619-241613-anti-cyclin-a1-cyclin-a2-antibody-epr18054-western-blot.jpg)
Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)All lanes : Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 52, 49 kDa
Observed band size: 49,52 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes5% NFDM/TBST: Blocking and diluting buffer.
-
![Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)](https://www.abcam.com/ps/products/185/ab185619/Images/ab185619-8-benefits-of-recombinant-antibodies.png)
Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
Protocols
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (13)
ab185619 has been referenced in 13 publications.
- Chen Q et al. Shenyan Kangfu tablet alleviates diabetic kidney disease through attenuating inflammation and modulating the gut microbiota. J Nat Med 75:84-98 (2021). PubMed: 32997272
- Kirstein N et al. Human ORC/MCM density is low in active genes and correlates with replication time but does not delimit initiation zones. Elife 10:N/A (2021). PubMed: 33683199
- Zheng P et al. KIF4A promotes the development of bladder cancer by transcriptionally activating the expression of CDCA3. Int J Mol Med 47:N/A (2021). PubMed: 33846765
- Ho CNQ et al. Simulated Microgravity Inhibits the Proliferation of Chang Liver Cells by Attenuation of the Major Cell Cycle Regulators and Cytoskeletal Proteins. Int J Mol Sci 22:N/A (2021). PubMed: 33925309
- Wang Y et al. Propofol activates AMPK to inhibit the growth of HepG2 cells in vitro and hepatocarcinogenesis in xenograft mouse tumor models by inducing autophagy. J Gastrointest Oncol 11:1322-1332 (2020). PubMed: 33457004
