Overview

  • Product name

  • Description

    Rabbit polyclonal to Cyclin A1
  • Host species

    Rabbit
  • Specificity

    ab133183 detects endogenous levels of total Cyclin A1 protein.
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide from the C-terminal region of Human Cyclin A1.

  • Positive control

    • SKOV3 cell extract; Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab133183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
  • Tissue specificity

    Very high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stage

    Expression increases in early G1 phase and reaches highest levels during the S and G2/M phases.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCN A1 antibody
    • CCNA 1 antibody
    • Ccna1 antibody
    • CCNA1_HUMAN antibody
    • Cyclin-A1 antibody
    • CyclinA1 antibody
    • G2/mitotic specific cyclin A1 antibody
    • MGC132235 antibody
    • MGC159139 antibody
    see all

Images

  • All lanes : Anti-Cyclin A1 antibody (ab133183) at 1/500 dilution

    Lane 1 : SKOV3 cell extract
    Lane 2 : SKOV3 cell extract with synthesized
    peptide

    Predicted band size: 52 kDa

  • Immunohistochemical analysis of paraffin embedded Human testis tissue labelling Cyclin A1 with ab133183 antibody at a concentration of 5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

This product has been referenced in:

  • Li F  et al. CEP55 promotes cell proliferation and inhibits apoptosis via the PI3K/Akt/p21 signaling pathway in human glioma U251 cells. Oncol Lett 15:4789-4796 (2018). Read more (PubMed: 29552118) »
See 1 Publication for this product

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