Overview

  • Product name
  • Description
    Rabbit polyclonal to Cyclin A1
  • Host species
    Rabbit
  • Specificity
    Detects endogenous levels of total Cyclin A1 protein.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • SKOV3 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab53699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA 1/40000.
IHC-P Use a concentration of 1 µg/ml.

Target

  • Function
    May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
  • Tissue specificity
    Very high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines.
  • Sequence similarities
    Belongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stage
    Expression increases in early G1 phase and reaches highest levels during the S and G2/M phases.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCN A1 antibody
    • CCNA 1 antibody
    • Ccna1 antibody
    • CCNA1_HUMAN antibody
    • Cyclin-A1 antibody
    • CyclinA1 antibody
    • G2/mitotic specific cyclin A1 antibody
    • MGC132235 antibody
    • MGC159139 antibody
    see all

Images

  • All lanes : Anti-Cyclin A1 antibody (ab53699) at 1/500 dilution

    Lane 1 : SKOV3 cell extract
    Lane 2 : SKOV3 cell extract with immunising peptide

    Predicted band size: 52 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab53699 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53699, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab53699 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53699, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Liu W  et al. Overexpression of non-SMC condensin I complex subunit G serves as a promising prognostic marker and therapeutic target for hepatocellular carcinoma. Int J Mol Med 40:731-738 (2017). Read more (PubMed: 28737823) »
  • Wang F  et al. Morusin inhibits cell proliferation and tumor growth by down-regulating c-Myc in human gastric cancer. Oncotarget 8:57187-57200 (2017). Read more (PubMed: 28915664) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium citrate pH 6.0
Sample
Mouse Tissue sections (Testes)
Specification
Testes
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Manju Sharma

Verified customer

Submitted Mar 19 2015

Answer

Thank you for contacting us.

Your credit note ID is XXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

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Answer

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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Answer

Thank you very much for confirming the details.

We are unsure about the identity of bands. Not many complaints are received for this antibody infact this is the second since December 2011 so protocol troubleshooting might help.

There is onepoint you may consider; the heavy and light chains of IP antibody. If rabbit antibody is used for IPing then you might observe 25, 55 kDa band corresponding to light and heavy chains however the band 80 kDa and 100 kDa is just not relevant what we should expect from this ab and in WB. So please check the antibody used for IP.

Secondly, It seems that you are Co-IPing the cyclin A1/ A2 with your protein of interest so the problem might be with the co-Ip procedure. I would suggest using whole hela cell lysates as positive control. If the whole cell lysates is already used (as ab input) then there is a band at 55 kda in 30 minutes exposure image which I presume is cyclin A1. If the whole lysates has notbeen used so I would suggest trying these also.

I am sure the above recommendations will be helpful. If however the results do not improves please contact me I will send you a replacement product.

Have a good weekend!

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Answer

Thanks you very much for your email. I am very sorry to hear that you are experiencing problems with this antibody.

I have checked the image and details you have kindly provided and in order to know more about the problem I have following questions;

- Could you fill the protocol details in the attached questionnaire?
- What is the name of cell line used?
- How the lysates was prepared? What sort of lysis buffer was used? Have you added any protease inhibitor in lysis buffer?
- Have you used the ab38 with IP lysate of ab70335? Could you please explain, how the IP lysate of ab70335 is compatible with ab38?
- Is it true both ab38 and ab70335 were used with same IP lysate?

Please send the details to us asap. Should you have any question please do not hesitate to contact me.

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