Key features and details
- Rabbit polyclonal to Cyclin A1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cyclin A1 antibody
See all Cyclin A1 primary antibodies
DescriptionRabbit polyclonal to Cyclin A1
SpecificityDetects endogenous levels of total Cyclin A1 protein.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide (Human)
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab53699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).|
FunctionMay be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
Tissue specificityVery high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines.
Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
Developmental stageExpression increases in early G1 phase and reaches highest levels during the S and G2/M phases.
- Information by UniProt
- CCN A1 antibody
- CCNA 1 antibody
- Ccna1 antibody
All lanes : Anti-Cyclin A1 antibody (ab53699) at 1/500 dilution
Lane 1 : SKOV3 cell extract
Lane 2 : SKOV3 cell extract with immunising peptide
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
ICC/IF image of ab53699 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53699, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab53699 has been referenced in 16 publications.
- Park S et al. Suppression of cathepsin a inhibits growth, migration, and invasion by inhibiting the p38 MAPK signaling pathway in prostate cancer. Arch Biochem Biophys 688:108407 (2020). PubMed: 32407712
- Wu L et al. LINP1 Promotes the Progression of Cervical Cancer by Scaffolding EZH2, LSD1 and DNMT1 to Inhibit the Expression of KLF2 and PRSS8. Biochem Cell Biol N/A:N/A (2020). PubMed: 32348690
- Leung WK et al. Evaluation of cyclin A1-specific T cells as a potential treatment for acute myeloid leukemia. Blood Adv 4:387-397 (2020). PubMed: 31985805
- Yao J et al. circGFRA1 Enhances NSCLC Progression by Sponging miR-188-3p. Onco Targets Ther 13:549-558 (2020). PubMed: 32021297
- Ghelli Luserna Di Rorà A et al. Synergism Through WEE1 and CHK1 Inhibition in Acute Lymphoblastic Leukemia. Cancers (Basel) 11:N/A (2019). PubMed: 31717700