Product nameAnti-Cyclin A1 antibody
See all Cyclin A1 primary antibodies
DescriptionRabbit polyclonal to Cyclin A1
SpecificityDetects endogenous levels of total Cyclin A1 protein.
Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide (Human)
- SKOV3 cell extracts.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab53699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).|
|IHC-P||Use a concentration of 1 µg/ml.|
FunctionMay be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
Tissue specificityVery high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines.
Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
Developmental stageExpression increases in early G1 phase and reaches highest levels during the S and G2/M phases.
- Information by UniProt
- CCN A1 antibody
- CCNA 1 antibody
- Ccna1 antibody
All lanes : Anti-Cyclin A1 antibody (ab53699) at 1/500 dilution
Lane 1 : SKOV3 cell extract
Lane 2 : SKOV3 cell extract with immunising peptide
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
ICC/IF image of ab53699 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53699, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab53699 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53699, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Liu W et al. Overexpression of non-SMC condensin I complex subunit G serves as a promising prognostic marker and therapeutic target for hepatocellular carcinoma. Int J Mol Med 40:731-738 (2017). Read more (PubMed: 28737823) »
- Wang F et al. Morusin inhibits cell proliferation and tumor growth by down-regulating c-Myc in human gastric cancer. Oncotarget 8:57187-57200 (2017). Read more (PubMed: 28915664) »