Anti-Cyclin A2 antibody (ab137769)
Key features and details
- Rabbit polyclonal to Cyclin A2
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Cyclin A2 antibody
See all Cyclin A2 primary antibodies -
Description
Rabbit polyclonal to Cyclin A2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig
-
Immunogen
Recombinant fragment corresponding to Human Cyclin A2 (internal sequence). Recombinant fragment encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.
Database link: P20248 -
Positive control
- 293T; A431; H1299; HeLaS3; HepG2; MOLT4; Raji and PC3 whole cell lysates; paraformaldehyde-fixed HeLa cells and paraffin-embedded NCIN87 xenograft.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/100 - 1/1000.
|
|
| IP |
1/100 - 1/500.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/1000. |
|
IP
1/100 - 1/500. |
Target
-
Function
Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER. - Information by UniProt
-
Database links
- Entrez Gene: 890 Human
- Entrez Gene: 12428 Mouse
- Entrez Gene: 100415929 Pig
- Entrez Gene: 114494 Rat
- Omim: 123835 Human
- SwissProt: P20248 Human
- SwissProt: P51943 Mouse
- Unigene: 58974 Human
see all -
Alternative names
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody (ab137769)
ab137769 detects Cyclin A2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin A2 protein stained by ab137769 diluted at 1/200.
Red: phalloidin, a cytoskeleton marker diluted at 1/200.
Blue: Hoechst 33342 staining. -
Western blot - Anti-Cyclin A2 antibody (ab137769)All lanes : Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : A375 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa -
Western blot - Anti-Cyclin A2 antibody (ab137769)All lanes : Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution
Lane 1 : SK-N-SH whole cell extract
Lane 2 : IMR32 whole cell extract
Lane 3 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa -
Western blot - Anti-Cyclin A2 antibody (ab137769)Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution + Neuro2A whole cell lysate at 30 µg
Predicted band size: 49 kDa -
Western blot - Anti-Cyclin A2 antibody (ab137769)Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg
Predicted band size: 49 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody (ab137769)Immunohistochemical analysis of paraffin embedded NCIN87 xenograft, labelling Cyclin A using ab137769 at 1/500 dilution. -
Immunoprecipitation - Anti-Cyclin A2 antibody (ab137769)ab137769 immunoprecipitates Cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate. A.) 30 μg 293T whole cell lysate. B.) Control with 2.5 μg of preimmune rabbit IgG. C.) Immunoprecipitation of Cyclin A2 protein by 2.5 μg of ab137769 10% SDS-PAGE: The immunoprecipitated Cyclin A2 protein was detected by ab137769 diluted at 1/1000.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab137769 has been referenced in 8 publications.
- Wang Y et al. Transducin-Like Enhancer of Split-1 Inhibits Malignant Behaviors in vitro and Predicts a Better Prognosis in Pancreatic Ductal Adenocarcinoma. Front Oncol 10:576 (2020). PubMed: 32432037
- Moses RL et al. Novel epoxy-tiglianes stimulate skin keratinocyte wound healing responses and re-epithelialization via protein kinase C activation. Biochem Pharmacol 178:114048 (2020). PubMed: 32446889
- Zhao C & Shen Q Overexpression of small ubiquitin-like modifier 2 ameliorates high glucose-induced reductions in cardiomyocyte proliferation via the transforming growth factor-ß/Smad pathway. Mol Med Rep 18:4877-4885 (2018). PubMed: 30280191
- Li F et al. LncRNA GAS5 Overexpression Reverses LPS-Induced Inflammatory Injury and Apoptosis Through Up-Regulating KLF2 Expression in ATDC5 Chondrocytes. Cell Physiol Biochem 45:1241-1251 (2018). PubMed: 29448248
- Yu W et al. Astragaloside IV reduces the hypoxia-induced injury in PC-12 cells by inhibiting expression of miR-124. Biomed Pharmacother 106:419-425 (2018). PubMed: 29990829
- Kosovec JE et al. CDK4/6 dual inhibitor abemaciclib demonstrates compelling preclinical activity against esophageal adenocarcinoma: a novel therapeutic option for a deadly disease. Oncotarget 8:100421-100432 (2017). PubMed: 29245989
- Oosthuysen WF et al. Neisseria meningitidis causes cell cycle arrest of human brain microvascular endothelial cells at S phase via p21 and cyclin G2. Cell Microbiol 18:46-65 (2016). PubMed: 26149128
- Yao Z et al. shRNA-mediated silencing of the RFC3 gene suppresses hepatocellular carcinoma cell proliferation. Int J Mol Med 36:1393-9 (2015). WB ; Human . PubMed: 26397132