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    cyclin-a2-antibody-ab137769.pdf

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Cell Biology Cell Cycle Cyclins
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Anti-Cyclin A2 antibody (ab137769)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (8)

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Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody (ab137769)
  • Western blot - Anti-Cyclin A2 antibody (ab137769)
  • Western blot - Anti-Cyclin A2 antibody (ab137769)
  • Western blot - Anti-Cyclin A2 antibody (ab137769)
  • Western blot - Anti-Cyclin A2 antibody (ab137769)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody (ab137769)
  • Immunoprecipitation - Anti-Cyclin A2 antibody (ab137769)

Key features and details

  • Rabbit polyclonal to Cyclin A2
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Protein
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Recombinant Human Cyclin A2 protein (ab126696)
Primary
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Anti-Cyclin A2 antibody [EPR17351] (ab181591)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Cyclin A2 antibody
    See all Cyclin A2 primary antibodies
  • Description

    Rabbit polyclonal to Cyclin A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Recombinant fragment corresponding to Human Cyclin A2 (internal sequence). Recombinant fragment encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.
    Database link: P20248

  • Positive control

    • 293T; A431; H1299; HeLaS3; HepG2; MOLT4; Raji and PC3 whole cell lysates; paraformaldehyde-fixed HeLa cells and paraffin-embedded NCIN87 xenograft.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cyclin A Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cyclins
    • Cyclin A Family
    • Cancer
    • Cell cycle
    • Cyclins
    • Cyclin A family
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Apolipoproteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human Cyclin A2 protein (ab126696)
  • Related Products

    • Recombinant Human Cyclin A2 protein (ab125570)
    • Recombinant Human Cyclin A2 protein (ab126696)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
IP
1/100 - 1/500.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
IP
1/100 - 1/500.

Target

  • Function

    Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stage

    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
  • Target information above from: UniProt accession P20248 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 890 Human
    • Entrez Gene: 12428 Mouse
    • Entrez Gene: 100415929 Pig
    • Entrez Gene: 114494 Rat
    • Omim: 123835 Human
    • SwissProt: P20248 Human
    • SwissProt: P51943 Mouse
    • Unigene: 58974 Human
    • Unigene: 4189 Mouse
    see all
  • Alternative names

    • CCN1 antibody
    • CCNA antibody
    • Ccna2 antibody
    • CCNA2_HUMAN antibody
    • Cyclin A2 antibody
    • Cyclin-A antibody
    • Cyclin-A2 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody (ab137769)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody (ab137769)

    ab137769 detects Cyclin A2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin A2 protein stained by ab137769 diluted at 1/200.
    Red: phalloidin, a cytoskeleton marker diluted at 1/200.
    Blue: Hoechst 33342 staining.

  • Western blot - Anti-Cyclin A2 antibody (ab137769)
    Western blot - Anti-Cyclin A2 antibody (ab137769)
    All lanes : Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : A375 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 49 kDa

  • Western blot - Anti-Cyclin A2 antibody (ab137769)
    Western blot - Anti-Cyclin A2 antibody (ab137769)
    All lanes : Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution

    Lane 1 : SK-N-SH whole cell extract
    Lane 2 : IMR32 whole cell extract
    Lane 3 : SK-N-AS whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 49 kDa

  • Western blot - Anti-Cyclin A2 antibody (ab137769)
    Western blot - Anti-Cyclin A2 antibody (ab137769)
    Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution + Neuro2A whole cell lysate at 30 µg

    Predicted band size: 49 kDa

  • Western blot - Anti-Cyclin A2 antibody (ab137769)
    Western blot - Anti-Cyclin A2 antibody (ab137769)
    Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg

    Predicted band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody (ab137769)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody (ab137769)
    Immunohistochemical analysis of paraffin embedded NCIN87 xenograft, labelling Cyclin A using ab137769 at 1/500 dilution.
  • Immunoprecipitation - Anti-Cyclin A2 antibody (ab137769)
    Immunoprecipitation - Anti-Cyclin A2 antibody (ab137769)

    ab137769 immunoprecipitates Cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate. A.) 30 μg 293T whole cell lysate. B.) Control with 2.5 μg of preimmune rabbit IgG. C.) Immunoprecipitation of Cyclin A2 protein by 2.5 μg of ab137769 10% SDS-PAGE: The immunoprecipitated Cyclin A2 protein was detected by ab137769 diluted at 1/1000.

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (8)

Publishing research using ab137769? Please let us know so that we can cite the reference in this datasheet.

ab137769 has been referenced in 8 publications.

  • Wang Y  et al. Transducin-Like Enhancer of Split-1 Inhibits Malignant Behaviors in vitro and Predicts a Better Prognosis in Pancreatic Ductal Adenocarcinoma. Front Oncol 10:576 (2020). PubMed: 32432037
  • Moses RL  et al. Novel epoxy-tiglianes stimulate skin keratinocyte wound healing responses and re-epithelialization via protein kinase C activation. Biochem Pharmacol 178:114048 (2020). PubMed: 32446889
  • Zhao C & Shen Q Overexpression of small ubiquitin-like modifier 2 ameliorates high glucose-induced reductions in cardiomyocyte proliferation via the transforming growth factor-ß/Smad pathway. Mol Med Rep 18:4877-4885 (2018). PubMed: 30280191
  • Li F  et al. LncRNA GAS5 Overexpression Reverses LPS-Induced Inflammatory Injury and Apoptosis Through Up-Regulating KLF2 Expression in ATDC5 Chondrocytes. Cell Physiol Biochem 45:1241-1251 (2018). PubMed: 29448248
  • Yu W  et al. Astragaloside IV reduces the hypoxia-induced injury in PC-12 cells by inhibiting expression of miR-124. Biomed Pharmacother 106:419-425 (2018). PubMed: 29990829
  • Kosovec JE  et al. CDK4/6 dual inhibitor abemaciclib demonstrates compelling preclinical activity against esophageal adenocarcinoma: a novel therapeutic option for a deadly disease. Oncotarget 8:100421-100432 (2017). PubMed: 29245989
  • Oosthuysen WF  et al. Neisseria meningitidis causes cell cycle arrest of human brain microvascular endothelial cells at S phase via p21 and cyclin G2. Cell Microbiol 18:46-65 (2016). PubMed: 26149128
  • Yao Z  et al. shRNA-mediated silencing of the RFC3 gene suppresses hepatocellular carcinoma cell proliferation. Int J Mol Med 36:1393-9 (2015). WB ; Human . PubMed: 26397132

Customer reviews and Q&As

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Western blot abreview for Anti-Cyclin A2 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Epithelial OVCAR cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
100 µg
Treatment
Perifosine 0 - 24 hr
Specification
Human Epithelial OVCAR cell line
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

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Verified customer

Submitted Apr 20 2017

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