Overview

  • Product name
  • Description
    Rabbit polyclonal to Cyclin A2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Recombinant fragment corresponding to Human Cyclin A2 (internal sequence). Recombinant fragment encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.
    Database link: P20248

  • Positive control
    • 293T; A431; H1299; HeLaS3; HepG2; MOLT4; Raji and PC3 whole cell lysates; paraformaldehyde-fixed HeLa cells and paraffin-embedded NCIN87 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab137769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.

Target

Images

  • ab137769 detects Cyclin A2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin A2 protein stained by ab137769 diluted at 1/200.
    Red: phalloidin, a cytoskeleton marker diluted at 1/200.
    Blue: Hoechst 33342 staining.

  • All lanes : Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : A375 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 49 kDa

  • All lanes : Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution

    Lane 1 : SK-N-SH whole cell extract
    Lane 2 : IMR32 whole cell extract
    Lane 3 : SK-N-AS whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 49 kDa

  • Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution + Neuro2A whole cell lysate at 30 µg

    Predicted band size: 49 kDa

  • Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg

    Predicted band size: 49 kDa

  • Immunohistochemical analysis of paraffin embedded NCIN87 xenograft, labelling Cyclin A using ab137769 at 1/500 dilution.
  • ab137769 immunoprecipitates Cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate. A.) 30 μg 293T whole cell lysate. B.) Control with 2.5 μg of preimmune rabbit IgG. C.) Immunoprecipitation of Cyclin A2 protein by 2.5 μg of ab137769 10% SDS-PAGE: The immunoprecipitated Cyclin A2 protein was detected by ab137769 diluted at 1/1000.

References

This product has been referenced in:
  • Yu W  et al. Astragaloside IV reduces the hypoxia-induced injury in PC-12 cells by inhibiting expression of miR-124. Biomed Pharmacother 106:419-425 (2018). Read more (PubMed: 29990829) »
  • Kosovec JE  et al. CDK4/6 dual inhibitor abemaciclib demonstrates compelling preclinical activity against esophageal adenocarcinoma: a novel therapeutic option for a deadly disease. Oncotarget 8:100421-100432 (2017). Read more (PubMed: 29245989) »
See all 3 Publications for this product

Customer reviews and Q&As

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Application
Western blot
Sample
Human Cell lysate - whole cell (Human Epithelial OVCAR cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
100 µg
Treatment
Perifosine 0 - 24 hr
Specification
Human Epithelial OVCAR cell line
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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Verified customer

Submitted Apr 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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