Overview

  • Product name
  • Description
    Rabbit polyclonal to Cyclin A2
  • Host species
    Rabbit
  • Specificity
    Antiserum will specifically react with a 60 kDa Cyclin A2 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin A from other species may also occur.
  • Tested applications
    Suitable for: WB, IP, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin A2.
    Database link: P20248

Properties

Applications

Our Abpromise guarantee covers the use of ab2097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 49 kDa.
IP 1/100.
ELISA 1/5000 - 1/20000.
ICC/IF 1/50. PubMed: 18083977

Target

Images

  • ab2097, staining Cyclin A in rabbit vascular smooth muscle cells, by Immunocytochemistry/ Immunofluorescence.

    Cells were either untreated (left) or treated with 10 µM ceramide (right), fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and incubated in blocking buffer (PBS, 2% skim milk, and 0.1% Tween-20) for 30 min at room temperature. Samples were incubated with primary antibody at 1/50 dilution for 1 hour at room temperature. An AlexaFluor®-conjugated goat anti-mouse IgG (1/1000) was used as the secondary antibody.

References

This product has been referenced in:
  • Yuan F & Wang W MicroRNA-802 suppresses breast cancer proliferation through downregulation of FoxM1. Mol Med Rep 12:4647-4651 (2015). Read more (PubMed: 26080894) »
  • Touati SA  et al. Cyclin A2 is required for sister chromatid segregation, but not separase control, in mouse oocyte meiosis. Cell Rep 2:1077-87 (2012). Read more (PubMed: 23122964) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry and your interest. The antibody you mentioned is a polyclonal antibody. Polyclonal antibodies consist of a mixed population of IgGs each of which will recognize a different epitope. Therefore, for polyclonal antibodies no epitope mapping experiments are carried out. I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

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Question
Answer

This antibody has only been tested on mammalian sources. It cross-reacts with rat and mouse. Presumably this molecule is so fundamental, that it is highly conserved in nature, but it hasn't been tested.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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