Key features and details
- Rabbit polyclonal to Cyclin A2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cyclin A2 antibody
See all Cyclin A2 primary antibodies
DescriptionRabbit polyclonal to Cyclin A2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Cyclin A2 aa 100-150. The exact sequence is proprietary.
Database link: P20248
- IP: Jurkat whole cell lysate. WB: HeLa, HEK-293T and Jurkat whole cell lysates.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226373 was affinity purified using an epitope specific to Cyclin A2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 49 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
- Information by UniProt
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
All lanes : Anti-Cyclin A2 antibody (ab226373) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 10 seconds
Cell lysates were prepared with NETN lysis buffer.
Cyclin A2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226373 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226373 at 0.4 µg/ml.
Lane 1: ab226373 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226373 has not yet been referenced specifically in any publications.