Overview

  • Product name

  • Description

    Rabbit polyclonal to Cyclin A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cyclin A2 aa 100-150. The exact sequence is proprietary.
    Database link: P20248

  • Positive control

    • IP: Jurkat whole cell lysate. WB: HeLa, HEK-293T and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab226373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stage

    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCN1 antibody
    • CCNA antibody
    • Ccna2 antibody
    • CCNA2_HUMAN antibody
    • Cyclin A2 antibody
    • Cyclin-A antibody
    • Cyclin-A2 antibody
    see all

Images

  • All lanes : Anti-Cyclin A2 antibody (ab226373) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 49 kDa


    Exposure time: 10 seconds


    Cell lysates were prepared with NETN lysis buffer.

  • Cyclin A2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226373 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226373 at 0.4 µg/ml.

    Lane 1: ab226373 IP in Jurkat whole cell lysate.

    Lane 2: Control IgG IP in Jurkat whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab226373 has not yet been referenced specifically in any publications.

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