Key features and details
- Rabbit polyclonal to Cyclin A2 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cyclin A2 antibody - C-terminal
See all Cyclin A2 primary antibodies
DescriptionRabbit polyclonal to Cyclin A2 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Pig, Rhesus monkey
Recombinant fragment within Human Cyclin A2 (C terminal). The exact sequence is proprietary.
Database link: P20248
- WB: Mouse brain tissue lysate. IHC-P: Human lung adenocarcinoma tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 49 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
- Information by UniProt
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
Anti-Cyclin A2 antibody - C-terminal (ab227277) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cyclin A2 (green) using ab227277 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Red: Phalloidin cytoskeletal marker.
Paraffin-embedded human lung adenocarcinoma tissue stained for Cyclin A2 with ab227277 at 1/250 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227277 has not yet been referenced specifically in any publications.