Product nameAnti-Cyclin A2 antibody [E399]
See all Cyclin A2 primary antibodies
DescriptionRabbit monoclonal [E399] to Cyclin A2
Specificityab32498 recognises Cyclin A2.
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC
Species reactivityReacts with: Human
Synthetic peptide within Human Cyclin A2 aa 100-200 (N terminal). The exact sequence is proprietary.
Epitopeab32498 reacts with an epitope located in the N terminal region of Cyclin A2
- WB: HeLa, K562 and Jurkat cell lysate. ICC/IF: HeLa cells.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 48 kDa.|
|ICC/IF||1/250 - 1/500.|
FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
- Information by UniProt
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
All lanes : Anti-Cyclin A2 antibody [E399] (ab32498) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking and dilution buffer: 5% NFDM/TBST.
The lower band is unidentified.
Immunofluorescent analysis of cyclin A expression in HeLa cells using 1/250 ab32498.
This product has been referenced in:
- Yang C et al. Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica. Int J Mol Med 43:1395-1405 (2019). Read more (PubMed: 30628649) »
- Iwahori S et al. Human cytomegalovirus-encoded viral cyclin-dependent kinase (v-CDK) UL97 phosphorylates and inactivates the retinoblastoma protein-related p107 and p130 proteins. J Biol Chem 292:6583-6599 (2017). Read more (PubMed: 28289097) »