Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E399] to Cyclin A2
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Product nameAnti-Cyclin A2 antibody [E399]
See all Cyclin A2 primary antibodies
DescriptionRabbit monoclonal [E399] to Cyclin A2
Specificityab32498 recognises Cyclin A2.
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC
Species reactivityReacts with: Human
Synthetic peptide within Human Cyclin A2 aa 100-200 (N terminal). The exact sequence is proprietary.
Epitopeab32498 reacts with an epitope located in the N terminal region of Cyclin A2
- WB: HeLa, K562 and Jurkat cell lysate. ICC/IF: HeLa cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab32498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 48 kDa.|
|ICC/IF||1/250 - 1/500.|
FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
- Information by UniProt
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
All lanes : Anti-Cyclin A2 antibody [E399] (ab32498) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking and dilution buffer: 5% NFDM/TBST.
The lower band is unidentified.
Immunofluorescent analysis of cyclin A expression in HeLa cells using 1/250 ab32498.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab32498 has been referenced in 9 publications.
- Yang C et al. Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica. Int J Mol Med 43:1395-1405 (2019). PubMed: 30628649
- Lopez-Martinez D et al. Phosphorylation of FANCD2 Inhibits the FANCD2/FANCI Complex and Suppresses the Fanconi Anemia Pathway in the Absence of DNA Damage. Cell Rep 27:2990-3005.e5 (2019). PubMed: 31167143
- Iwahori S & Kalejta RF Phosphorylation of transcriptional regulators in the retinoblastoma protein pathway by UL97, the viral cyclin-dependent kinase encoded by human cytomegalovirus. Virology 512:95-103 (2017). PubMed: 28946006
- Iwahori S et al. Human cytomegalovirus-encoded viral cyclin-dependent kinase (v-CDK) UL97 phosphorylates and inactivates the retinoblastoma protein-related p107 and p130 proteins. J Biol Chem 292:6583-6599 (2017). PubMed: 28289097
- Zhang YY et al. Iodine regulates G2/M progression induced by CCL21/CCR7 interaction in primary cultures of papillary thyroid cancer cells with RET/PTC expression. Mol Med Rep 14:3941-6 (2016). PubMed: 27574129
- Wei X et al. Proteomics-based identification of the tumor suppressor role of aminoacylase 1 in hepatocellular carcinoma. Cancer Lett 351:117-25 (2014). WB ; Human . PubMed: 24846301
- Wu Z et al. The inhibitory role of Mir-29 in growth of breast cancer cells. J Exp Clin Cancer Res 32:98 (2013). WB . PubMed: 24289849
- Yajima H et al. The complexity of DNA double strand breaks is a critical factor enhancing end-resection. DNA Repair (Amst) 12:936-46 (2013). PubMed: 24041488
- Edel MJ et al. A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells. Cell Commun Signal 9:8 (2011). WB . PubMed: 21481269