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RecombinantRabMAb

Recombinant Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)

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Western blot - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
  • Western blot - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
  • Flow Cytometry - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
  • Immunoprecipitation - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
  • Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E399] to Cyclin A2 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

HRP Unconjugated

Overview

  • Product name

    Anti-Cyclin A2 antibody [E399] - BSA and Azide free
    See all Cyclin A2 primary antibodies

  • Description

    Rabbit monoclonal [E399] to Cyclin A2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises Cyclin A2.

  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    ab247261 reacts with an epitope located in the N terminal region of Cyclin A2

  • Positive control

    • IP: K-562 whole cell lysate. WB: HeLa untreated, HeLa treated G1/S phase, K562 and HeLa cell lysate

  • General notes

    ab247261 is the carrier-free version of ab32498.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.

  • Sequence similarities

    Belongs to the cyclin family. Cyclin AB subfamily.

  • Developmental stage

    Accumulates steadily during G2 and is abruptly destroyed at mitosis.

  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
  • Information by UniProt

  • Database links

    • Alternative names

      • CCN1 antibody
      • CCNA antibody
      • Ccna2 antibody
      • CCNA2_HUMAN antibody
      • Cyclin A2 antibody
      • Cyclin-A antibody
      • Cyclin-A2 antibody
      see all

    Images

    • Western blot - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
      Western blot - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
      All lanes : Anti-Cyclin A2 antibody [E399] (ab32498) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate; Untreated, asynchronous cells (ab136811)
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate; G1/S arrested cells (thymidine treatment) (ab136811)
      Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate; G2/M arrested cells (sequential thymidine and nocodazole treatments) (ab136811)

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 48 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?



      Cyclin A2 is down regulated at the G2/M phase.

    • Western blot - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
      Western blot - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
      All lanes : Anti-Cyclin A2 antibody [E399] (ab32498) at 1/2000 dilution (Purified)

      Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 48 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?



      The smaller band is due to a tissue-specific splice variant(PMID 22745723). The band of 35kDa maybe the cleaved form(PMID: 12176996).

    • Flow Cytometry - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
      Flow Cytometry - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)

      This data was developed using ab247261, the same antibody clone in a different buffer formulation.
      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Cyclin A2 with Purified ab247261 at 1:30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
      This data was developed using ab32498, the same antibody clone in a different buffer formulation.
      Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cyclin A2 with Purified ab32498 at 1/50 dilution (5.66 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunoprecipitation - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
      Immunoprecipitation - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)

      This data was developed using ab32498, the same antibody clone in a different buffer formulation.
      Purified ab32498 at 1/20 dilution (1µg) immunoprecipitating Cyclin A2 in K-562 whole cell lysate.
      Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
      Lane 2 (+): ab32498 + K-562 whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32498 in K-562 whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 50 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
      This data was developed using ab32498, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of cyclin A expression in HeLa cells using 1/250 ab32498.
    • Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
      Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247261? Please let us know so that we can cite the reference in this datasheet.

    ab247261 has not yet been referenced specifically in any publications.

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