Recombinant
RabMAb

Recombinant Anti-Cyclin A2 antibody [E399] (HRP) (ab193598)

Overview

  • Product name

    Anti-Cyclin A2 antibody [E399] (HRP)
    See all Cyclin A2 primary antibodies
  • Description

    Rabbit monoclonal [E399] to Cyclin A2 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cyclin A2 aa 100-200 (N terminal). The exact sequence is proprietary.

  • Epitope

    ab32498 reacts with an epitope located in the N terminal region of Cyclin A2
  • Positive control

    • WB: HeLa whole cell lysate. IHC-P: FFPE human breast adenocarcinoma
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab193598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).

Target

  • Function

    Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stage

    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCN1 antibody
    • CCNA antibody
    • Ccna2 antibody
    • CCNA2_HUMAN antibody
    • Cyclin A2 antibody
    • Cyclin-A antibody
    • Cyclin-A2 antibody
    see all

Images

  • Anti-Cyclin A2 antibody [E399] (HRP) (ab193598) at 1/1000 dilution + HeLa whole cell lysate (ab150035) at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab193598 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • IHC image of Cyclin A2 staining in a section of formalin-fixed paraffin-embedded human breast adenocarcinoma*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab193598 at a working dilution of 1/100. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and manual) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab193598 has not yet been referenced specifically in any publications.

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