Name: Anti-Cyclin A2 antibody [EPR17351]
Brand: RabMAb
SKU: ab181591
Rating: 5 (1 reviews)

Product name

Anti-Cyclin A2 antibody [EPR17351]
See all Cyclin A2 primary antibodies
Description

Rabbit monoclonal [EPR17351] to Cyclin A2
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, IHC-P, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Mouse Cyclin A2 aa 1-200. The exact sequence is proprietary.
Database link: P51943

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Positive control
- WB: Jurkat, C6, RAW 264.7 and PC-12 whole cell lysates; HeLa Untreated, asynchronous cells, HeLa G1/S arrested cells (thymidine treatment) and HeLa G2/M arrested cells (sequential thymidine and nocodazole treatments) cell lysates; Human tonsil and fetal kidney lysates; Rat spleen lysate. IHC-P: Human tonsil, Human cervix carcinoma, rat colon and mouse endometrium tissues. ICC/IF: HeLa cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17351
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab181591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/500.
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).

Function

Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
Sequence similarities

Belongs to the cyclin family. Cyclin AB subfamily.
Developmental stage

Accumulates steadily during G2 and is abruptly destroyed at mitosis.
Cellular localization

Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
Target information above from: UniProt accession P20248 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 890 Human
- Entrez Gene: 12428 Mouse
- Entrez Gene: 114494 Rat
- Omim: 123835 Human
- SwissProt: P20248 Human
- SwissProt: P51943 Mouse
- Unigene: 58974 Human
- Unigene: 4189 Mouse
- Unigene: 13094 Rat
see all
Alternative names
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
- CCNA2_HUMAN antibody
- Cyclin A2 antibody
- Cyclin-A antibody
- Cyclin-A2 antibody
see all

Western blot - Anti-Cyclin A2 antibody [EPR17351] (ab181591)

Anti-Cyclin A2 antibody [EPR17351] (ab181591) at 1/20000 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [EPR17351] (ab181591)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR17351]- Loading Control (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab181591 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150120) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [EPR17351]- Loading Control (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] (ab181591)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on germinal center cells of Human tonsil tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Western blot - Anti-Cyclin A2 antibody [EPR17351] (ab181591)

All lanes : Anti-Cyclin A2 antibody [EPR17351] (ab181591) at 1/20000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate; Untreated, asynchronous cells (ab136811)
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate; G1/S arrested cells (thymidine treatment) (ab136811)
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate; G2/M arrested cells (sequential thymidine and nocodazole treatments) (ab136811)

Lysates/proteins at 1 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated or Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Cyclin A2 is down regulated at the G2/M phase.
Western blot - Anti-Cyclin A2 antibody [EPR17351] (ab181591)

Anti-Cyclin A2 antibody [EPR17351] (ab181591) + Human tonsil lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Cyclin A2 antibody [EPR17351] (ab181591)

Anti-Cyclin A2 antibody [EPR17351] (ab181591) at 1/2000 dilution + Human fetal kidney lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
The smaller band is due to a tissue-specific splice variant, PMID 22745723.
Western blot - Anti-Cyclin A2 antibody [EPR17351] (ab181591)

All lanes : Anti-Cyclin A2 antibody [EPR17351] (ab181591) at 1/2000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Cyclin A2 antibody [EPR17351] (ab181591)

Anti-Cyclin A2 antibody [EPR17351] (ab181591) at 1/2000 dilution + Rat spleen lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
The smaller band is due to a tissue-specific splice variant, PMID 22745723.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] (ab181591)

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on some tumor cells in Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] (ab181591)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on a proportion of epithelial cells in rat colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] (ab181591)

Immunohistochemical analysis of paraffin-embedded mouse endometrium tissue labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on a proportion of epithelial cells in mouse endometrial tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Datasheet

This product has been referenced in:

Yang B et al. H2A.Z regulates tumorigenesis, metastasis and sensitivity to cisplatin in intrahepatic cholangiocarcinoma. Int J Oncol 52:1235-1245 (2018). Read more (PubMed: 29532867) »
Xu L et al. Morphological adaptation of sheep's rumen epithelium to high-grain diet entails alteration in the expression of genes involved in cell cycle regulation, cell proliferation and apoptosis. J Anim Sci Biotechnol 9:32 (2018). Read more (PubMed: 29686866) »

See all 12 Publications for this product

Publishing research using ab181591? Please let us know so that we can cite the reference in this datasheet.

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Anti-Cyclin A2 antibody [EPR17351] (ab181591)

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