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RecombinantRabMAb

Recombinant Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)
  • Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17351] to Cyclin A2 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Unconjugated

Overview

  • Product name

    Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free
    See all Cyclin A2 primary antibodies

  • Description

    Rabbit monoclonal [EPR17351] to Cyclin A2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, C6, RAW 264.7 and PC-12 whole cell lysates; HeLa Untreated, asynchronous cells, HeLa G1/S arrested cells (thymidine treatment) and HeLa G2/M arrested cells (sequential thymidine and nocodazole treatments) cell lysates; Human tonsil and fetal kidney lysates; Rat spleen lysate. IHC-P: Human tonsil, Human cervix carcinoma, rat colon and mouse endometrium tissues. ICC/IF: HeLa cells.

  • General notes

    ab231164 is the carrier-free version of ab181591.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab231164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on some tumor cells in Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181591).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on a proportion of epithelial cells in rat colon tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181591).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)

    Immunohistochemical analysis of paraffin-embedded mouse endometrium tissue labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on a proportion of epithelial cells in mouse endometrial tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181591).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR17351]- Loading Control (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab181591 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [EPR17351]- Loading Control (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181591).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)

    This IHC data was generated using the same anti-Cyclin A2 antibody clone, EPR17351, in a different buffer formulation (cat# ab181591).

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on germinal center cells of Human tonsil tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)

    This ICC data was generated using the same anti-Cyclin A2 antibody clone, EPR17351, in a different buffer formulation (cat# ab181591).

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR17351]- Loading Control (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab181591 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [EPR17351]- Loading Control (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.

  • Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)
    Anti-Cyclin A2 antibody [EPR17351] - BSA and Azide free (ab231164)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab231164? Please let us know so that we can cite the reference in this datasheet.

ab231164 has not yet been referenced specifically in any publications.

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