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RecombinantRabMAb

Recombinant Anti-Cyclin A2 antibody [Y193] (ab32386)

  • Datasheet
Reviews (6) Submit a question References (19)

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Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)
  • Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)
  • Immunoprecipitation - Anti-Cyclin A2 antibody [Y193] (ab32386)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [Y193] (ab32386)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [Y193] (ab32386)
  • Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)
  • Anti-Cyclin A2 antibody [Y193] (ab32386)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y193] to Cyclin A2
  • Suitable for: IHC-P, IP, WB, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

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Recombinant Human Cyclin A2 protein (ab126696)
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Secondary
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Overview

  • Product name

    Anti-Cyclin A2 antibody [Y193]
    See all Cyclin A2 primary antibodies
  • Description

    Rabbit monoclonal [Y193] to Cyclin A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, HEK293, NIH/3T3 cell lysates. IHC-P: Skin and colon carcinoma. ICC/IF: MCF7 cells. IP: HeLa cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y193
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cyclin A Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cyclins
    • Cyclin A Family
    • Cancer
    • Cell cycle
    • Cyclins
    • Cyclin A family
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Apolipoproteins

Associated products

  • Alternative Versions

    • Anti-Cyclin A2 antibody [Y193] - BSA and Azide free (ab167392)
    • Alexa Fluor® 568 Anti-Cyclin A2 antibody [Y193] (ab210980)
    • Alexa Fluor® 594 Anti-Cyclin A2 antibody [Y193] (ab211581)
    • Alexa Fluor® 647 Anti-Cyclin A2 antibody [Y193] (ab211805)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Cyclin A2 protein (ab126696)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/40 - 1/50.
WB (2)
1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

For unpurified, use 1/1000.

ICC/IF (2)
1/100 - 1/250.
Notes
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/40 - 1/50.
WB
1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

For unpurified, use 1/1000.

ICC/IF
1/100 - 1/250.

Target

  • Function

    Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stage

    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
  • Target information above from: UniProt accession P20248 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 890 Human
    • Entrez Gene: 12428 Mouse
    • Omim: 123835 Human
    • SwissProt: P20248 Human
    • SwissProt: P51943 Mouse
    • Unigene: 58974 Human
    • Unigene: 4189 Mouse
    • Alternative names

      • CCN1 antibody
      • CCNA antibody
      • Ccna2 antibody
      • CCNA2_HUMAN antibody
      • Cyclin A2 antibody
      • Cyclin-A antibody
      • Cyclin-A2 antibody
      see all

    Images

    • Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)
      Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)
      Anti-Cyclin A2 antibody [Y193] (ab32386) at 1/10000 dilution (purified) + NIH/3T3 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 48 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)
      Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)
      All lanes : Anti-Cyclin A2 antibody [Y193] (ab32386) at 1/10000 dilution (purified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : HEK293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 48 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunoprecipitation - Anti-Cyclin A2 antibody [Y193] (ab32386)
      Immunoprecipitation - Anti-Cyclin A2 antibody [Y193] (ab32386)

      ab32386 (purified) at 1/40 immunoprecipitating Cyclin A2 in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 55 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP Detection Reagent (ab131366) was used for detection (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [Y193] (ab32386)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [Y193] (ab32386)

      Immunofluorescence staining of MCF7 cells with purified ab32386 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4 % PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32386 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [Y193] (ab32386)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [Y193] (ab32386)
      Immunohistochemical staining of paraffin embedded human colon carcinoma with purified ab32386 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)
      Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)
      Anti-Cyclin A2 antibody [Y193] (ab32386) at 1/1000 dilution (unpurified) + HeLa cell lysate

      Predicted band size: 48 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?

    • Anti-Cyclin A2 antibody [Y193] (ab32386)
      Anti-Cyclin A2 antibody [Y193] (ab32386)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (19)

    Publishing research using ab32386? Please let us know so that we can cite the reference in this datasheet.

    ab32386 has been referenced in 19 publications.

    • Herbert KJ  et al. Targeting TOPK sensitises tumour cells to radiation-induced damage by enhancing replication stress. Cell Death Differ 28:1333-1346 (2021). PubMed: 33168956
    • Ishiguro KI  et al. MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells. Dev Cell N/A:N/A (2020). ICC/IF ; Mouse . PubMed: 32032549
    • Wang L  et al. The transcriptional regulator MEIS2 sets up the ground state for palatal osteogenesis in mice. J Biol Chem 295:5449-5460 (2020). PubMed: 32169905
    • Alfieri C  et al. A unique binding mode of Nek2A to the APC/C allows its ubiquitination during prometaphase. EMBO Rep 21:e49831 (2020). PubMed: 32307883
    • Liu J  et al. Embryonic stem cell microenvironment enhances proliferation of human retinal pigment epithelium cells by activating the PI3K signaling pathway. Stem Cell Res Ther 11:411 (2020). PubMed: 32967731
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

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    1-6 of 6 Abreviews

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin A2 antibody [Y193]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (C42B Prostate cancer cell line)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    C42B Prostate cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Dimitra Kalamida

    Verified customer

    Submitted Mar 02 2020

    Western blot abreview for Anti-Cyclin A2 antibody [Y193]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (C42B Prostate cancer cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    C42B Prostate cancer cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Dimitra Kalamida

    Verified customer

    Submitted Dec 25 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cyclin A2 antibody [Y193]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast cancer cell line xenograft)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
    Permeabilization
    No
    Specification
    Breast cancer cell line xenograft
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 23 2015

    Immunocytochemistry abreview for Anti-Cyclin A2 antibody [Y193]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (HeLa)
    Permeabilization
    No
    Specification
    HeLa
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    Submitted Oct 26 2015

    Western blot abreview for Anti-Cyclin A2 antibody [Y193]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (10% acrylamide)
    Loading amount
    50 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MRS. Vanessa Maillet

    Verified customer

    Submitted Aug 27 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin A2 antibody [Y193]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    No
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    Submitted Aug 20 2014

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