Recombinant Anti-Cyclin A2 antibody [Y193] (ab32386)
![Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.com/ps/products/32/ab32386/Images/ab32386-259957-anti-cyclin-a2-antibody-y193-western-blot.jpg "Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y193] to Cyclin A2
- Suitable for: IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cyclin A2 antibody [Y193]
See all Cyclin A2 primary antibodies -
Description
Rabbit monoclonal [Y193] to Cyclin A2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2, HEK293, NIH/3T3 cell lysates. IHC-P: Skin and colon carcinoma. ICC/IF: MCF7 cells. IP: HeLa cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y193 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| IP |
1/40 - 1/50.
|
|
| WB | (2) |
1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
For unpurified, use 1/1000. |
| ICC/IF | (2) |
1/100 - 1/250.
|
| Notes |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/40 - 1/50. |
|
WB
1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa). For unpurified, use 1/1000. |
|
ICC/IF
1/100 - 1/250. |
Target
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Function
Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER. - Information by UniProt
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Database links
- Entrez Gene: 890 Human
- Entrez Gene: 12428 Mouse
- Omim: 123835 Human
- SwissProt: P20248 Human
- SwissProt: P51943 Mouse
- Unigene: 58974 Human
- Unigene: 4189 Mouse
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Alternative names
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
see all
Images
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Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)Anti-Cyclin A2 antibody [Y193] (ab32386) at 1/10000 dilution (purified) + NIH/3T3 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.com/ps/products/32/ab32386/Images/ab32386-259956-anti-cyclin-a2-antibody-y193-western-blot.jpg)
Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)All lanes : Anti-Cyclin A2 antibody [Y193] (ab32386) at 1/10000 dilution (purified)
Lane 1 : HepG2 cell lysate
Lane 2 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunoprecipitation - Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.com/ps/products/32/ab32386/Images/ab32386-259951-anti-cyclin-a2-antibody-y193-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cyclin A2 antibody [Y193] (ab32386)ab32386 (purified) at 1/40 immunoprecipitating Cyclin A2 in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 55 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP Detection Reagent (ab131366) was used for detection (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.com/ps/products/32/ab32386/Images/ab32386-259945-anti-cyclin-a2-antibody-y193-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [Y193] (ab32386)Immunofluorescence staining of MCF7 cells with purified ab32386 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4 % PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32386 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.com/ps/products/32/ab32386/Images/ab32386-259941-anti-cyclin-a2-antibody-y193-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [Y193] (ab32386)Immunohistochemical staining of paraffin embedded human colon carcinoma with purified ab32386 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.com/ps/products/32/ab32386/Images/ab32386-44671-anti-cyclin-a2-antibody-y193-western-blot.jpg)
Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)Anti-Cyclin A2 antibody [Y193] (ab32386) at 1/1000 dilution (unpurified) + HeLa cell lysate
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
![Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.com/ps/products/32/ab32386/Images/ab32386-8-benefits-of-recombinant-antibodies.png)
Anti-Cyclin A2 antibody [Y193] (ab32386)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (19)
ab32386 has been referenced in 19 publications.
- Herbert KJ et al. Targeting TOPK sensitises tumour cells to radiation-induced damage by enhancing replication stress. Cell Death Differ 28:1333-1346 (2021). PubMed: 33168956
- Ishiguro KI et al. MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells. Dev Cell N/A:N/A (2020). ICC/IF ; Mouse . PubMed: 32032549
- Wang L et al. The transcriptional regulator MEIS2 sets up the ground state for palatal osteogenesis in mice. J Biol Chem 295:5449-5460 (2020). PubMed: 32169905
- Alfieri C et al. A unique binding mode of Nek2A to the APC/C allows its ubiquitination during prometaphase. EMBO Rep 21:e49831 (2020). PubMed: 32307883
- Liu J et al. Embryonic stem cell microenvironment enhances proliferation of human retinal pigment epithelium cells by activating the PI3K signaling pathway. Stem Cell Res Ther 11:411 (2020). PubMed: 32967731
