Anti-Cyclin B1 antibody (ab2096)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-Cyclin B1 antibody
See all Cyclin B1 primary antibodies -
Description
Rabbit polyclonal to Cyclin B1 -
Host species
Rabbit -
Specificity
Antiserum will specifically react with a 62 kDa Cyclin B1 protein. No reaction was observed against other related cyclins. -
Tested applications
Suitable for: WB, IP, ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Fusion protein corresponding to Cyclin B1 aa 1-433. full length fusion protein
Database link: P14635
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide -
Purity
Whole antiserum -
Purification notes
This product was prepared from monospecific antiserum by delipidation and defibrination. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab2096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa). | |
| IP | Use at an assay dependent dilution. | |
| ELISA | 1/500 - 1/2000. |
Target
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Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
Post-translational
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 891 Human
- Entrez Gene: 268697 Mouse
- Entrez Gene: 25203 Rat
- Omim: 123836 Human
- SwissProt: P14635 Human
- SwissProt: P24860 Mouse
- SwissProt: P30277 Rat
- Unigene: 23960 Human
see all -
Alternative names
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
see all
Datasheets and documents
References
This product has been referenced in:
- Jin LZ et al. Silencing SUMO2 promotes protection against degradation and apoptosis of nucleus pulposus cells through p53 signaling pathway in intervertebral disc degeneration. Biosci Rep 38:N/A (2018). Read more (PubMed: 29700214) »
- Kotecha M et al. Inhibition of DNA binding of the NF-Y transcription factor by the pyrrolobenzodiazepine-polyamide conjugate GWL-78. Mol Cancer Ther 7:1319-28 (2008). WB ; Mouse . Read more (PubMed: 18483319) »
