Anti-Cyclin B1 antibody (ab215436)
Key features and details
- Rabbit polyclonal to Cyclin B1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclin B1 antibody
See all Cyclin B1 primary antibodies -
Description
Rabbit polyclonal to Cyclin B1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Mouse Cyclin B1 aa 350-450 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P24860 -
Positive control
- WB: HeLa, U937, U251 and K562 cell lysates. IHC-P: Human colon carcinoma tissue. . Flow Cyt (Intra): HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/20 - 1/100.
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WB |
1/100 - 1/1000. Predicted molecular weight: 48 kDa.
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IHC-P |
1/100 - 1/500.
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Notes |
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Flow Cyt (Intra)
1/20 - 1/100. |
WB
1/100 - 1/1000. Predicted molecular weight: 48 kDa. |
IHC-P
1/100 - 1/500. |
Target
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Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
Post-translational
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 891 Human
- Entrez Gene: 268697 Mouse
- Entrez Gene: 25203 Rat
- Omim: 123836 Human
- SwissProt: P14635 Human
- SwissProt: P24860 Mouse
- SwissProt: P30277 Rat
- Unigene: 23960 Human
see all -
Alternative names
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
see all
Images
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Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution + HeLa cell lysates
Predicted band size: 48 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling Cyclin B1 with ab215436 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining
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Intracellular flow cytometric analysis of HeLa cells labeling Cyclin B1with ab215436 at 1/100 dilution for 60 minutesat room temperature, followed by incubation with aGoat Anti-Rabbit IgG (H+L) Alexa Fluor 488 conjugated secondary antibody.
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Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution + U937 cell lysates
Predicted band size: 48 kDa -
Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution + U251 cell lysates
Predicted band size: 48 kDa -
Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution + K562 cell lysates
Predicted band size: 48 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab215436 has been referenced in 6 publications.
- Li S & Tu H Psoralen inhibits the proliferation and promotes apoptosis through endoplasmic reticulum stress in human osteosarcoma cells. Folia Histochem Cytobiol 60:101-109 (2022). PubMed: 35257357
- Peng W et al. The intercorrelation among CCT6A, CDC20, CCNB1, and PLK1 expressions and their clinical value in papillary thyroid carcinoma prognostication. J Clin Lab Anal 36:e24609 (2022). IHC ; Human . PubMed: 35838025
- Canu V et al. Aberrant transcriptional and post-transcriptional regulation of SPAG5, a YAP-TAZ-TEAD downstream effector, fuels breast cancer cell proliferation. Cell Death Differ 28:1493-1511 (2021). PubMed: 33230261
- Su H et al. YBX1 regulated by Runx3-miR-148a-3p axis facilitates non-small-cell lung cancer progression. Cell Signal 85:110049 (2021). PubMed: 34082012
- Liu Q et al. Brucine inhibits proliferation of glioblastoma cells by targeting the G-quadruplexes in the c-Myb promoter. J Cancer 12:1990-1999 (2021). PubMed: 33753997
- Pan YQ et al. Effect of MT2A on apoptosis and proliferation in HL60 cells. Int J Med Sci 18:2910-2919 (2021). PubMed: 34220318