Product nameAnti-Cyclin B1 antibody - N-terminal
See all Cyclin B1 primary antibodies
DescriptionRabbit polyclonal to Cyclin B1 - N-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human Cyclin B1 aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: P14635
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226397 was affinity purified using an epitope specific to Cyclin B1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 48 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
All lanes : Anti-Cyclin B1 antibody - N-terminal (ab226397) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
Cyclin B1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226397 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226397 at 1 µg/ml.
Lane 1: ab226397 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
ab226397 has not yet been referenced specifically in any publications.