Overview

  • Product name

    Anti-Cyclin B1 antibody - N-terminal
    See all Cyclin B1 primary antibodies
  • Description

    Rabbit polyclonal to Cyclin B1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human Cyclin B1 aa 1-50 (N terminal). The exact sequence is proprietary.
    Database link: P14635

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 48 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-Cyclin B1 antibody - N-terminal (ab226397) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 48 kDa


    Exposure time: 10 seconds


    Lysates prepared using NETN lysis buffer.

  • Cyclin B1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226397 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226397 at 1 µg/ml.

    Lane 1: ab226397 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 1 second.

References

ab226397 has not yet been referenced specifically in any publications.

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