Anti-Cyclin B1 antibody [V152] - BSA and Azide free (ab186926)

Overview

  • Product name

    Anti-Cyclin B1 antibody [V152] - BSA and Azide free
    See all Cyclin B1 primary antibodies
  • Description

    Mouse monoclonal [V152] to Cyclin B1 - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    Cyclin B1 expression is restricted to a specific short period of the cell cycle with cyclin B1 expression detected earlier and peaking in concentration before cyclin B2 expression.

  • Tested applications

    Suitable for: IHC-P, IHC (PFA fixed), ICC/IF, Flow Cyt, SDS-PAGE, IHC-FoFr, IHC-Fr, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Fusion protein within Hamster Cyclin B1. The exact sequence is proprietary.

  • Positive control

    • WB: HAP1 Wild type, HeLa and HEK-293T whole cell lysate, IHC-P: Human tonsil tissue. Flow: HeLa cells
  • General notes

    ab186926 is the carrier-free version of ab72. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab182842 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab186926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
IHC (PFA fixed) 1/400.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
SDS-PAGE 1/1500.
IHC-FoFr Use a concentration of 1 - 2 µg/ml.
IHC-Fr Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

Abcam recommends using 5% Milk as the blocking agent.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Essential for the control of the cell cycle at the G2/M (mitosis) transition.
    • Sequence similarities

      Belongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stage

      Accumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Post-translational
      modifications

      Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • Database links

    • Alternative names

      • CCNB 1 antibody
      • CCNB antibody
      • ccnb1 antibody
      • CCNB1_HUMAN antibody
      • Cyclin B1 antibody
      • G2 mitotic specific cyclin B1 antibody
      • G2/mitotic-specific cyclin-B1 antibody
      see all

    Images

    • Lanes:
      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Cyclin B1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HEK293 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab72 observed at 55 kDa. Red - loading control, ab181602, observed at 37 kDa.

      ab72 detected the expected band for Cyclin B1 in wild type HAP1 cells and the band was not seen in Cyclin B1 knockout HAP1 cells. Wild-type and Cyclin B1 knockout samples were subjected to SDS-PAGE. Ab72 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab72).

    • ab72 staining Cyclin B1 in human tonsil tissue - formalin-fixed paraffin-embedded section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab72, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab72).

    • Flow cytometry analysis of HeLa cells stained with ab72 (green line). Cell harvesting/tissue preparation method - Trypsin/EDTA. Sample Buffer: Complete Media then PBS. Fixation: Formaldehyde. Permeabilization: 90% methanol. Dilution: 1/50. Incubation time: 1 hour at 23°C, diluent: PBS/10% goat serum. Secondary antibody: Goat anti-mouse FITC at 1/1000 dilution. 100,000 cells were stained, non-specific IgG antibody - negative control (purple histogram)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab72).

    References

    ab186926 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab186926.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up