Anti-Cyclin B1 antibody [V92.1] (ab18221)

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Key features and details

  • Mouse monoclonal [V92.1] to Cyclin B1
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Cyclin B1 antibody [V92.1]
    See all Cyclin B1 primary antibodies

  • Description

    Mouse monoclonal [V92.1] to Cyclin B1

  • Host species

    Mouse

  • Specificity

    V92.1 can immunoprecipitate active CDK1/cyclin B1 complexes.

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Full length protein (Hamster).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab18221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 1 µg/ml.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Essential for the control of the cell cycle at the G2/M (mitosis) transition.

    • Sequence similarities

      Belongs to the cyclin family. Cyclin AB subfamily.

    • Developmental stage

      Accumulates steadily during G2 and is abruptly destroyed at mitosis.

    • Post-translational
      modifications

      Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.

    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CCNB 1 antibody
      • CCNB antibody
      • ccnb1 antibody
      • CCNB1_HUMAN antibody
      • Cyclin B1 antibody
      • G2 mitotic specific cyclin B1 antibody
      • G2/mitotic-specific cyclin-B1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [V92.1] (ab18221)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [V92.1] (ab18221)
      ICC/IF image of ab18221 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18221, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References (4)

    Publishing research using ab18221? Please let us know so that we can cite the reference in this datasheet.

    ab18221 has been referenced in 4 publications.

    • Jin X  et al. Identification of key pathways and genes in lung carcinogenesis. Oncol Lett 16:4185-4192 (2018). PubMed: 30250533
    • Sekine E  et al. Spatiotemporal dynamics of HSV genome nuclear entry and compaction state transitions using bioorthogonal chemistry and super-resolution microscopy. PLoS Pathog 13:e1006721 (2017). PubMed: 29121649
    • Garner AP  et al. Delta MEKK3:ER* activation induces a p38 alpha/beta 2-dependent cell cycle arrest at the G2 checkpoint. Oncogene 21:8089-104 (2002). PubMed: 12444545
    • Minemoto Y  et al. Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53. Exp Cell Res 262:37-48 (2001). PubMed: 11120603

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