Anti-Cyclin B1 antibody [V92.1] (ab18221)
![Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [V92.1] (ab18221)](https://www.abcam.com/ps/products/18/ab18221/Images/ab18221-27525-anti-cyclin-b1-antibody-v921-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [V92.1] (ab18221)")
Key features and details
- Mouse monoclonal [V92.1] to Cyclin B1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cyclin B1 antibody [V92.1]
See all Cyclin B1 primary antibodies -
Description
Mouse monoclonal [V92.1] to Cyclin B1 -
Host species
Mouse -
Specificity
V92.1 can immunoprecipitate active CDK1/cyclin B1 complexes. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein (Hamster).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
V92.1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
| Notes |
|---|
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
Post-translational
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 891 Human
- Omim: 123836 Human
- SwissProt: P14635 Human
- Unigene: 23960 Human
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Alternative names
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [V92.1] (ab18221)ICC/IF image of ab18221 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18221, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab18221 has been referenced in 4 publications.
- Jin X et al. Identification of key pathways and genes in lung carcinogenesis. Oncol Lett 16:4185-4192 (2018). PubMed: 30250533
- Sekine E et al. Spatiotemporal dynamics of HSV genome nuclear entry and compaction state transitions using bioorthogonal chemistry and super-resolution microscopy. PLoS Pathog 13:e1006721 (2017). PubMed: 29121649
- Garner AP et al. Delta MEKK3:ER* activation induces a p38 alpha/beta 2-dependent cell cycle arrest at the G2 checkpoint. Oncogene 21:8089-104 (2002). PubMed: 12444545
- Minemoto Y et al. Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53. Exp Cell Res 262:37-48 (2001). PubMed: 11120603
