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Cell Biology Cell Cycle Cyclins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)

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Immunoprecipitation - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
  • Flow Cytometry - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
  • Flow Cytometry - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
  • Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y106] to Cyclin B1 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Human

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Protein
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Recombinant Human Cyclin B1 protein (ab128445)
Conjugation
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APC Conjugation Kit - Lightning-Link® (ab201807)

View more associated products

Overview

  • Product name

    Anti-Cyclin B1 antibody [Y106] - BSA and Azide free
    See all Cyclin B1 primary antibodies
  • Description

    Rabbit monoclonal [Y106] to Cyclin B1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab156447 is the carrier-free version of ab32053. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab156447 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y106
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cyclin B Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cyclins
    • Cyclin B Family
    • Cancer
    • Cell cycle
    • Cyclins
    • Cyclin B family

Associated products

  • Alternative Versions

    • HRP Anti-Cyclin B1 antibody [Y106] (ab193977)
    • PE Anti-Cyclin B1 antibody [Y106] (ab210615)
    • Alexa Fluor® 555 Anti-Cyclin B1 antibody [Y106] (ab214381)
    • Anti-Cyclin B1 antibody [Y106] (ab32053)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Cyclin B1 protein (ab128445)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab156447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stage

    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Post-translational
    modifications

    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Target information above from: UniProt accession P14635 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 891 Human
    • Omim: 123836 Human
    • SwissProt: P14635 Human
    • Unigene: 23960 Human
    • Alternative names

      • CCNB 1 antibody
      • CCNB antibody
      • ccnb1 antibody
      • CCNB1_HUMAN antibody
      • Cyclin B1 antibody
      • G2 mitotic specific cyclin B1 antibody
      • G2/mitotic-specific cyclin-B1 antibody
      see all

    Images

    • Immunoprecipitation - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
      Immunoprecipitation - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)

      ab32053 (purified) at 1:20 dilution (2μg) immunoprecipitating Cyclin B1 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate.

      Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
      Lane 2 (+): ab32053 & Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32053 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32053).

    • Flow Cytometry - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
      Flow Cytometry - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)

      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cyclin B1 with purified ab32053 at 1:400 dilution (1 µg/ml) (red). Cells were fixed with 80% Methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Left). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). Cells were pre-treated with 20μg/ml RNaseA for 30min to minimize the binding between PI and RNA.Then intracellular stained with ab32053 and PI.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32053).

    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)

      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cyclin B1 with Purified ab32053 at 1:100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32053).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical carcinoma tissue sections labeling Cyclin B1 with Purified ab32053 at 1:250 dilution (1.47 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32053).

    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)This image is courtesy of an Abreview submitted by Stephanie Hilss.

      Unpurified ab32053 staining Cyclin B1 in the U2OS cell line from human by ICC/IF (Immunocytochemistry/immunofluorescence).Cells were fixed with formaldehyde,permeabilized with 1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at 37°C.Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32053).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)

      Unpurified ab32053 at a 1:100 dilution staining Human cyclin B1 in human skin carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32053).

    • Flow Cytometry - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
      Flow Cytometry - Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)

      Overlay histogram showing Jurkat cells stained with unpurified ab32053 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32053, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32053).

    • Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)
      Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab156447? Please let us know so that we can cite the reference in this datasheet.

    ab156447 has not yet been referenced specifically in any publications.

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