Anti-Cyclin B1 (phospho S126) antibody (ab3488)
Key features and details
- Rabbit polyclonal to Cyclin B1 (phospho S126)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Cyclin B1 (phospho S126) antibody
See all Cyclin B1 primary antibodies -
Description
Rabbit polyclonal to Cyclin B1 (phospho S126) -
Host species
Rabbit -
Specificity
Minimal reactivity is expected with the non-phosphorylated form of the protein. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Dog, Chimpanzee -
Immunogen
Synthetic peptide corresponding to Human Cyclin B1 aa 120-131 (phospho S126).
Sequence: ILVDTASpPSPME
Database link: P14635 -
General notes
This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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Notes |
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WB
1/100 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
Target
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Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
Post-translational
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 461835 Chimpanzee
- Entrez Gene: 101836169 Hamster
- Entrez Gene: 891 Human
- Entrez Gene: 268697 Mouse
- Entrez Gene: 25203 Rat
- Omim: 123836 Human
- SwissProt: P37882 Hamster
- SwissProt: P14635 Human
see all -
Alternative names
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
see all
Images
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All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : H23 whole cell lysate
Lane 3 : Hepatocarcinoma (Hep3b) cell lysate
Lane 4 : Glioblastoma (T98G) cell lysate
Lane 5 : B cell lymphoblast (Daudi) cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Exposure time: 20 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab3488 has been referenced in 1 publication.
- Müllers E et al. Residual Cdk1/2 activity after DNA damage promotes senescence. Aging Cell 16:575-584 (2017). PubMed: 28345297