Key features and details
- Rabbit polyclonal to Cyclin B1 (phospho S126)
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cyclin B1 (phospho S126) antibody
See all Cyclin B1 primary antibodies
DescriptionRabbit polyclonal to Cyclin B1 (phospho S126)
SpecificityMinimal reactivity is expected with the non-phosphorylated form of the protein.
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Dog, Chimpanzee
This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesThis antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
Our Abpromise guarantee covers the use of ab3488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : H23 whole cell lysate
Lane 3 : Hepatocarcinoma (Hep3b) cell lysate
Lane 4 : Glioblastoma (T98G) cell lysate
Lane 5 : B cell lymphoblast (Daudi) cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Exposure time: 20 seconds
ab3488 has been referenced in 1 publication.
- Müllers E et al. Residual Cdk1/2 activity after DNA damage promotes senescence. Aging Cell 16:575-584 (2017). PubMed: 28345297