Overview

  • Product name

    Anti-Cyclin B1 (phospho S147) antibody
    See all Cyclin B1 primary antibodies
  • Description

    Rabbit polyclonal to Cyclin B1 (phospho S147)
  • Host species

    Rabbit
  • Specificity

    ab131502 detects endogenous levels of Cyclin B1 only when phosphorylated at serine 147
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin B1 aa 145-149 (phospho S147) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    AFSpDV


    Database link: P14635

  • Positive control

    • HeLa cells, MDA cells treated with anisomycin, Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 48 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

Images

  • All lanes : Anti-Cyclin B1 (phospho S147) antibody (ab131502) at 1/500 dilution

    Lane 1 : Untreated MDA cell lysate
    Lane 2 : Lysate of MDA cells treated with anisomycin

    Predicted band size: 48 kDa

  • Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131502 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelled with ab131502 at 1/50 dilution (left) and ab131502 preincubated with blocking peptide (right).

References

ab131502 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131502.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up