Key features and details
- Rabbit polyclonal to Cyclin B1 (phospho S147)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cyclin B1 (phospho S147) antibody
See all Cyclin B1 primary antibodies
DescriptionRabbit polyclonal to Cyclin B1 (phospho S147)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Cyclin B1 (phospho S147). Immunogen range is aa 121-170.
Database link: P14635
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab60986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately >48 kDa (predicted molecular weight: 48 kDa).|
FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
All lanes : Anti-Cyclin B1 (phospho S147) antibody (ab60986) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells treated with UV light (15 mins).
Lane 2 : Extracts from Jurkat cells treated with UV light (15 mins) and the immunising phosphopeptide.
Predicted band size: 48 kDa
Observed band size: >48 kDa why is the actual band size different from the predicted?
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab60986 at 1/50-1/100 dilution, with or without the immunising phosphopeptide.
ab60986 has been referenced in 2 publications.
- Goan YG et al. Kinome-Wide Screening with Small Interfering RNA Identified Polo-like Kinase 1 as a Key Regulator of Proliferation in Oral Cancer Cells. Cancers (Basel) 11:N/A (2019). PubMed: 31387297
- Holt JE et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010). WB ; Mouse . PubMed: 20223764