Product nameAnti-Cyclin B2 antibody [X29.2]
See all Cyclin B2 primary antibodies
DescriptionMouse monoclonal [X29.2] to Cyclin B2
SpecificityReacts with most cyclin Bs.
Tested applicationsSuitable for: IHC-P, ICC/IF, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human, Xenopus laevis
Full length protein (Xenopus laevis).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A/G purified
Our Abpromise guarantee covers the use of ab18250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 45 kDa. The antibody may give a high background if the concentration of the antibody is above 2µg/ml.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
- Information by UniProt
- ccnb2 antibody
- CCNB2_HUMAN antibody
- CycB2 antibody
All lanes : Anti-Cyclin B2 antibody [X29.2] (ab18250) at 2 µg/ml
Lane 1 : Whole cell lysate of Mitotic Hela cells (arrested by the spindle checkpoint due to depolymerization of microtubles by exposure to Nocodazole)
Lane 2 : Whole cell lysate of Interphase Hela Cells, forced to exit mitosis by exposure to a Cdk1 inhibitor (exit from mitosis results in the degradation of Cyclin B2)
All lanes : HRP-Conjugated Goat anti-Mouse diluted 1/20000
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Additional bands at: 90 kDa (possible non-specific binding)
Exposure time: 25 minutes
The gel was denaturing. Blocked with a 5% milk solution for 2 hours at 24°C.
The primary antibody was diluted in 5%Milk/Tris-Buffered Saline/0.05%Tween20 and incubated for 16 hours at 4°C.
IHC image of ab18250 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18250, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Overlay histogram showing HeLA cells stained with ab18250 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18250, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Möckel MM et al. Xenopus laevis Kif18A is a highly processive kinesin required for meiotic spindle integrity. Biol Open 6:463-470 (2017). Read more (PubMed: 28228376) »
- Dupré AI et al. The greatwall kinase is dominant over PKA in controlling the antagonistic function of ARPP19 in Xenopus oocytes. Cell Cycle 16:1440-1452 (2017). Read more (PubMed: 28722544) »