Key features and details
- Rabbit polyclonal to Cyclin B2/CCNB2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cyclin B2/CCNB2 antibody
See all Cyclin B2/CCNB2 primary antibodies
DescriptionRabbit polyclonal to Cyclin B2/CCNB2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Cyclin B2/CCNB2 aa 46-348.
Database link: O95067
- H1299 whole cell lysate; HeLa cells; H1299 xenograft
General notesKeep as a concentrated solution.
This product was previously labelled as Cyclin B2
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 45 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
- Information by UniProt
- ccnb2 antibody
- CCNB2_HUMAN antibody
- CycB2 antibody
Anti-Cyclin B2/CCNB2 antibody (ab155596) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS PAGE
Immunofluorescent analysis of methanol-fixed HeLa cells labeling Cyclin B2/CCNB2 with ab155596 at 1/500 dilution. Right panel co-stained with Hoechst 33343.
Cells were fixed in ice-cold methanol for 5 minutes.
Immunohistochemical analysis of paraffin-embedded H1299 xenograft tissue labeling Cyclin B2/CCNB2 with ab155596 at 1/500 dilution.
ab155596 has not yet been referenced specifically in any publications.